东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

In situ observation of C60（C（COOH）2）2 interacting with living cells using fluorescence microscopy

ISSN号：1006-446X
期刊名称：《广东微量元素科学》
时间：0
分类：TB383[一般工业技术—材料科学与工程]
作者机构：[1]Laboratory for Bio-Environmental Health Sciences of Nanoscale Materials, Institute of High Energy of Physics, Chinese Academy of Sciences, Beijing 100049, China, [2]Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China, [3]National Center for Nanoscience and Technology, Beijing 100080, China, [4]Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
相关基金：Acknowledgements The authors thank Prof. Liu Yuanfang of Peking University for his valuable suggestions and discussions. This work was supported by the National Natural Science Foundation of China （Grant Nos. 10490180 and 90406024）, the 0utstanding Young Natural Scientist Foundation （Grant Nos. 10525524 and 20225516）, the Ministry of Science and Technology （Grant No. 2005CB724703）, and the Directionality Program of the Chinese Academy of Sciences （Grant No. KJCX-SW-H12）.

作者： YE Chang[1], CHEN Chunying[1], CHEN Zhen[1], MENG Huan[1], XING Li[1], JIANG Yaxin[2], YUAN Hui[1], XING Gengmei[1], ZHAO Feng[1], ZHAO Yuliang[1], CHAI Zhifang[1], FANG Xiaohong[2], HAN Dong[3], CHEN Long[3], WANG Chen[3], WEI Taotao[4]

关键词： C60(C(COOH)2)2, 原位观察, 真实时间成象, 球壳状碳分子, 细胞膜, 荧光标记, fluorescence microscopy, real time imaging, fullerene derivative C60（C（COOH）2）2, cross cellular membrane, fluorescence labeling.

中文摘要：

在 nanoparticles 和生活房间之间的相互作用被荧光显微镜学的一种成像技术调查。为这个目的， C_(60 )derivativeC_(60 )(C (COOH )_2 )_2，为中央神经系统的退化疾病的一个治疗学的代理人，被综合，净化并且描绘。它和细胞的膜的生活房间和穿入的相互作用在用实时成像技术，和它的 potentialcytotoxicity 学习的 situ 被流动 cy-tometry 也检验。结果显示 _2 容易装的 C_(60 )(C (COOH )_2 ) 进入房间，并且被荧光黄标记主要位于细胞质。而且， C_(60 )(C (COOH )_2 )_2 能带不能穿过细胞的膜进房间的分子，因为荧光黄混合物本身不能进入房间或遵守膜。在从 1x10 ~的集中(-2) 到 1x10 ~ 2 mg/L， _2 不显示出的 C_(60 )(C (COOH )_2 ) 任何可检测的 cytotoxicity。

英文摘要：

The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C60 derivative C60（C（COOH）2）2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C60（C（COOH）2）2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60（C（COOH）2）2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1×10^-2 to 1×10^2 mg/L, C60（C（COOH）2）2 does not show any detectable cytotoxicity.

同期刊论文项目