中文摘要：

[目的]探讨灵菌红素对铜绿微囊藻生长的抑制作用,以及不同抑制状态下微囊藻毒素水平的变化。[方法]培养铜绿微囊藻至对数期后染毒,应用细胞计数结合流式细胞仪分析0.156-5.000μg/m L 6个剂量灵菌红素对铜绿微囊藻生长的抑制效果。1.25、2.50、5.00μg/m L灵菌红素染毒24、48 h后,对活性氧（ROS）、超氧化物歧化酶（SOD）、DNA含量以及细胞内外的微囊藻毒素进行检测。[结果]灵菌红素染毒24 h对铜绿微囊藻的半数效应浓度（EC50）为2.76μg/m L（95%可信区间是0.96-7.91μg/m L）。2.50、5.00μg/m L灵菌红素染毒24 h后,ROS水平分别为8.47、19.60,均高于对照组;SOD活性分别为14.24、5.31 U/m L,均较对照组降低。染毒后细胞膜的完整性存在不同程度的受损。1.25、2.50、5.00μg/m L灵菌红素染毒24 h后,铜绿微囊藻的直径分别为2.72、2.73、2.75μm,明显高于正常细胞（2.54μm）;DNA含量亦增加,分别为正常值的1.08、1.18、1.32倍。灵菌红素抑制微囊藻毒素LR的产生,2.50μg/m L时抑制效果最显著,染毒72 h后细胞微囊藻毒素LR含量较对照组降低23.80%。[结论]灵菌红素能诱导细胞氧化损伤和增殖抑制,抑制铜绿微囊藻生长。同时,灵菌红素能抑制微囊藻毒素LR的产生。

英文摘要：

[ Objective ] To explore the inhibitory effects of prodigiosin on the growth of Microcystis aeruginosa （M. aeruginosa） and the production of microeystins during different inhibition processes. [ Methods ] M. aeruginosa was cultivated to logarithmic phase, and then the effects of prodigiosin on growth were examined by cell counting and flow cytometry assay after exposure to six doses of prodigiosin between 0.156 and 5.000 tag/mL. The contents of reactive oxygen species （ROS）, the activities of superoxide dismutase （SOD）, the contents of DNA, and the intracellular and extracellular mierocystins of M. aeruginosa were detected respectively after 24h and 48h exposure to 1.25, 2.50, and 5.00μg/mL prodigiosin. [ Results ] The half maximal effective concentration （EC50） value of prodigiosin for growth inhibition of M. aeruginosa was 2.76 μg/mL （95% confidence interval, 0.96-7.91 μg/mL）. The content of ROS was 8.47 and 19.60 respectively, and the activity of SOD was 14.24 and 5.31 U/mL respectively after 24 h exposure to 2.50 and 5.00 μg/mL prodigiosin; both were decreased compared to the control group. Damaged membrane integrity was observed in varying degrees after exposure to different doses of prodigiosin. The cell diameter of M. aeruginosa was 2.72, 2.73, and 2.75 μm respectively and was larger than that of normal cells （2.54 μm） after 24h exposure to 1.25, 2.50, and 5.00 μg/mL prodigiosin; the average DNA content also elevated as 1.08, 1.18, 1.32 times of normal cells. Furthermore, the microcystin-LR production of M. aeruginosa was inhibited after exposure to prodigiosin; the most remarkable inhibitory effect was shown when 2.50 μg/mL prodigiosin was added and the content of microcystin-LR was reduced by 23.80% after 72 h. [ Conclusion Prodigiosin could induce cell oxidative damage and proliferation inhibition to inhibit the growth of M. aeruginosa. In addition, prodigiosin could reduce the production of microcystin-LR.