中文摘要：

前期研究发现小凹蛋白-1（caveolin-1,Cav-1）和细胞外钙敏感受体（extracellular Ca2＋-sensing receptor,CaR）在人脐静脉内皮细胞（human umbilical vein endothelial cells,HUVECs）膜上有共定位,且Cav-1下调CaR介导的钙内流。本实验探讨HUVECs中Cav-1与CaR的亚细胞定位,可为进一步机制研究提供理论依据。以3~4代HU-VECs随机分为2组：（1）对照组;（2）甲基-β-环糊精（MβCD）处理组,分别采用蔗糖密度梯度离心和Western blot技术提取含小凹膜碎片和检测Cav-1和CaR蛋白的表达。结果显示,（1）MβCD处理组HUVECs细胞中Cav-1和CaR蛋白的表达与对照组相比无差异（P〉0.05）。（2）MβCD处理组小凹区Cav-1和CaR蛋白的表达低于对照组（P〈0.05）。由此可知,Cav-1和CaR蛋白共定位于HUVECs的小凹区,MβCD可抑制两者在小凹的共定位。

英文摘要：

Our previous results demonstrated that extracellular Ca2＋-sensing receptor（CaR） and caveolin-1（Cav-1） co-localized on the plasma localization of Cav-1 and CaR in HUVECs,which provide a theoretical basis for further functional studies.Methods：The third or fourth passage of HUVECs were randomly divided into two groups：（1） Control group;（2）methyl-β-cyelodextrin（MβCD） group.We isolated caveolae-enriched membrane fractions from MβCD-treated HUVECs by TritonX-100 sucrose density gradient fractionation and detected the expressions of Cav-1 and CaR protein by Western blot,respectively.Results：（1）The expression of Cav-1 and CaR proteins between control group and MβCD group was no difference（P〉0.05）.（2）The expressions of Cav-1 and CaR proteins in caveolae after MβCD treated was significantly reduced（P〈0.05）.Conclusion：The present study suggests that CaR and Cav-1 co-localize in caveolae in HUVECS,MβCD reduces the co-localization of Cav-1 and CaR in caveolae.