中文摘要：

利用高效液相色谱-二极管阵列检测法（HPLC—DAD）研究了不同含水量、不同样品浓度及不同反应时间对原花青素降解（正丁醇／HCl法）产物花青定及反应副产物的影响。木榄花样品经70％丙酮溶液提取，利用SephadexLH．20柱纯化后得到原花青素样品。配制不同含水量和不同样品浓度的反应溶液，充分震荡后进行正丁醇／HCl法降解。用HPLC—DAD分析不同实验条件下得到的反应产物及副产物。结果表明，反应体系在含水量5％～15％的条件下，转化产物随着反应体系含水量的增加，花青定的转化率也随之增加，副产物的转化率却随之减少，总转化率（即花青定加上副产物的总转化量）呈增加趋势；在反应体系含水量大于15％时，随含水量的增加，转化产物含量呈现下降的趋势；增加反应体系中样品含量并没有明显增加转化产物的转化率，副产物的转化率相对稳定；不同反应时间处理得到的转化产物经HPLC．DAD分析并未发现转化产物花青定和副产物表现出明显的变化规律，总转化率相对稳定。

英文摘要：

The effect of different water content, sample concentration and reaction time on anthocyanidin from degradation of proanthocyanidin and side products was studied by high performance liquid chromatography diode array detector （HPLC-DAD）. Proanthocyanidin was extracted from calyces of Bruguiera gymnorrhiza by 70% acetone solvent, purified by Sephadex LH-20 column chromatography, and was prepared to reaction sol- vents with different water content and sample concentration, then was analyzed with the butanol/HC1 method. In addition, the products and side products were characterized by HPLC-DAD. The yield of anthocyanidin was increased with the increasing the water content （from 5% to 15% ） of reaction solvents, but the yield of side products was declined. When the water content was above 15%, the yield of anthocyanidin and side products was decreased with the increasing the water content. However, there is no great effect of sample concentration and reaction time on the yield of anthocyanidin and side products. HPLC-DAD is a powerful method for the analysis of the content and structure unit of proanthocyanidin.