中文摘要：

采用紫外光光刻、硅蚀刻及软光刻技术分别制备了阴性光刻胶SU-8和聚乳酸及聚羟基乙酸共聚物（PLGA）微小凹图式。以人神经母细胞瘤细胞SH—SY5Y与三维微结构进行复合，采用扫描电子显微镜、光学显微镜及caicein荧光染色评价所加工图式的结构形态及细胞的生长行为。实验制备出名义直径约100μm、高纵横比（接近或大于1）并可叠加微通道连接的SU-8及PLGA三维微小凹图式。所加工的图式透明，符合三维细胞生物传感器的光学检测的需要。细胞培养结果表明，该结构尺寸的图式能引导在三维环境神经细胞的排布、神经突起延伸及形态分化。

英文摘要：

UV lithography, silicon etching and soft lithography were adopted to fabricate microwell patterns with SU-8,a negative photoresist, and poly（DL-lactide-co-glycolide） （PLGA）. Patterns were integrated with SH-SY5Y human neuroblastoma cells. SEM, light microscopy as well as calcein fluorescence staining were used to evaluate the pattern structure profiles and cell growth behaviors. The results showed that our fabricated patterns had a nominal well diameter of around 100μm and a high aspect ratio of around or even greater than 1 with or without microchannel connection. The patterns were also transparent and thus rendered suitable for optical detection as a 3-D format scaffold of cell based biosensors. Cell culture experiment results demonstrated that patterns of such dimension can not only guide cell alignmen and neural extensions, but also affect the cell morphological differentiation.