中文摘要：

对生防链霉菌Ⅲ-61产生抗真菌活性物质的摇瓶发酵工艺进行了研究。利用正交试验设计优化了发酵培养基组分，其最适配方为黄豆粉1．5％，蛋白胨0．3％，蔗糖1．0％，淀粉1．3％，磷酸二氢钾0．02％，硫酸镁0．025％，氯化钠0．5％，配咸水溶液，调pH至7～7．4，加碳酸钙1％。通过单因素试验，筛选获得了最优培养条件组合：液体种龄24h，接种量5％～10％，500mL摇瓶培养基装量为80mL，摇床转速240r／min，培养温度31℃，发酵周期96～120h。此优化的发酵培养基与发酵条件的组合昕得菌株Ⅲ-61发酵液对主要靶标黄瓜灰霉病菌的抑菌圈直径达49．5mm，较优化前提高了45．59％。

英文摘要：

A study on the fermentation process in shake flask to produce antifungal active substance by Streptomyces Ⅲ-61, a biocontrol strain isolated from the forest soil in a suburb of Beijing, was launched. The fermentation medium was optimized by orthogonal experiment. The result showed that the optimal liquid medium was composed of 1.5% soybean meal, 0.3% peptone, 1.0% sucrose, 1.3% soluble starch, 0.02% KH2PO4, 0.025% MgSO4·7H2O and 0.5% NaCl. The initial pH of the solution was adjusted to 7.0--7.4, then 1% CaCO3 was added. The culture conditions were screened with mono - factor test, and the most suitable technical parameters were summed up as follows： the liquid seed was cultured for 24 hours, the fermentation was carried out in 500mL erlenmeyer flask with 80mL production medium inoculated with 5%--10% seed culture and incubated at 240 r/min, 31℃ for 96--120 hours. The inhibition zone diameter against Botrytis cinerea up to 49.5mm by the germfree filtrate from fermented broth of strain Ⅲ-61 obtained with the optimized fermentation process, which is 45.59% higher than that produced by the basic medium and initial fermentation process.