中文摘要：

为了研究不同温度、初始p H、碳源和氮源在固体培养条件下对粗毛纤孔菌菌丝生长的影响,对以上4个因素进行单因素试验,从中选出3个最优的水平进行正交试验。结果表明,粗毛纤孔菌的最适生长温度为30℃;最适初始p H为6.5;最适碳源为木糖;最适氮源为酵母浸粉。在玉米、高粱和小麦3种基质中,对原种培养基进行了筛选,实验结果表明高粱和玉米在接种后的第1天和第2天开始萌发,且可更好地促进粗毛纤孔菌菌丝的生长。对粗毛纤孔菌栽培料的3种配方进行了筛选,实验结果表明配方：木屑78%、稻壳2%、玉米粉17%、蔗糖1%、石灰1%、石膏1%、含水量60%–62%的比例为栽培种培养料可使粗毛纤孔菌菌丝在接种28d后长满菌袋,且生长旺盛,可较快地培育出子实体。

英文摘要：

Effect of different carbon source, nitrogen source, initial p H and temperature on mycelial growth of Inonotus hispidus under the solid culture condition was investigated by orthogonal experiments. The optimal cultivation conditions for mycelial growth were 30°C for temperature, 6.5 for initial p H, xylose for the best carbon source and the yeast extract powder for the best nitrogen source. The natural substrates＇ test indicated corn and sorghum significantly enhanced the initial and continuous growth. The best substrate formulation is composed of sawdust 78%, rice husk 2%, corn flour 17%, sugar 1%, lime 1%, gypsum 1% and water 60%–62%, which well supports the mycelial growth and sackful within 28 days, and accelerates primordium formation and fruitage.