中文摘要：

背景：研究表明脂多糖对子宫内膜细胞具有损伤作用。目的：通过噻唑蓝和流式细胞术观察黄芩苷对脂多糖诱导的子宫内膜细胞活性、周期和凋亡率的影响。设计、时间及地点：以细胞为对象的分组对比观察实验，于2005-11/2007-03在湖南中医药大学完成。材料：子宫内膜组织标本3例，来自中南大学湘雅医学院第三附属医院和湖南省妇幼保健医院因不孕症行诊断性刮宫术，或子宫肌瘤行子宫全切除的患者；黄芩提取物由长沙艾菌生物科技开发有限公司提供，黄芩苷含量≥90％，由湖南中医药大学药学院药物制剂室制备黄芩苷药物，原液浓度为80g／L。方法：体外培养人子宫内膜细胞。将细胞随机分为空白组、黄芩苷组（8，0．8，0．08，0．008g／L）。根据细胞活性最好的黄芩苷浓度以下实验将细胞随机分为空白组、脂多糖组、脂多糖＋黄芩苷组（0．08g／L）。主要观察指标：①采用噻唑蓝法检测黄芩苷对子宫内膜细胞活性的影响。②采用流式细胞术检测黄芩苷对脂多糖诱导子宫内膜细胞凋亡率及周期的影响。结果：①噻唑蓝法检测显示黄芩苷组除0．008g／L外，其余各浓度对子宫内膜细胞活性均有抑制作用，选择0．08g／L进行后续实验。②流式细胞术检测显示黄芩苷干预后G0/G1和G2/M期细胞百分比较脂多糖组明显降低，S期明显增高，细胞凋亡率明显降低。结论：黄芩苷可以使G0/G1期细胞被诱导进入S期，从而促进细胞的增殖，降低了脂多糖诱导的细胞凋亡。

英文摘要：

BACKGROUND： Many studies show that lipopolysaccharide （LPS） can damage endometrial cells. OBJECTIVE： To detect the effects of baicalirl on the activity, apoptosis and cell cycle of endometrial cells induced by LPS through MTT and flow cytometry. DESIGN, TIME AND SETTING： The comparative observation trial was performed at Hunan University of Traditional Chinese Medicine from November 2005 to March 2007. MATERIALS： Three samples of endomertrial tissues were obtained from patients, who underwent dilatation and curettage because of barrenness or uterectomy because of hysteromyoma in the Third Hospital of Xiangya of Center South University and Hunan Woman and Child Healthcare Hospital. Extracts of scutellaria were provided from Changsha Active Ingredients Group, Inc., and the content of baicalirl was ≥90%. Baicalin was prepared by Department of Pharmaceutical Preparation, College of Pharmacy, Hunan University of Traditional Chinese Medicine. METHODS： Endometrium cells were cultured in vitro, and randomly divided into blank group and baicalin group （8, 0.8, 0.08, 0.008 g/L）. According to the best density of baicalin, cells were randomly divided into blank group, LPS group, and LPS ＋baicalirl （0.08 g/L） group. MAIN OUTCOME MEASURES： （1）The effect of baicalin on the activity of endometrial cells was detected through MTT; （2）Cell Cycle and apoptosis of endometrial cells induced by LPS was detected using flow cytometry. RESULTS： MTT showed that beside 0.008 g/L baicalirl group, the activity of endometrial cells was depressed by the other concentration groups. 0.08 g/L baicalin was selected for subsequent experiment. Flow cytometry showed that baicalirl could significantly decrease the percentage of cells in G0/G1 and G2/M-phase, while increase the percentage of cells in S-phase, and decrease apoptosis compared with LPS group. CONCLUSION： Baicalin can induce cells in G0/G1 phase into S-phase to promote cell proliferation and decrease LPS-induced cell apoptosis.