中文摘要：

为了系统研究镉诱导华溪蟹不同组织（肝胰腺，腮）金属硫蛋白（MT）表达及镉蓄积状况，并探讨二者之间的关系，采用体外镉暴露法处理华溪蟹，用镉-血红蛋白饱和法和火焰原子吸收分光光度法（AAS）分别测定华溪蟹肝胰腺及腮中镉蓄积及MT表达含量．结果显示，暴露期间动物死亡率低于9．2％，体重未见显著变化．不同浓度镉暴露下，华溪蟹体内镉蓄积及金属硫蛋白表达具有明显组织差异，且呈现一定的时间-效应与剂量-效应关系．鳃中镉蓄积量高于肝胰腺且最高达到（15．41±3．21）μg·g^-1而肝胰腺中MT表达量高于鳃，最高达到（97．18±11．83）μg·g-1通过鳃及肝胰腺中MT的镉结合率计算，显示肝胰腺中的镉可完全被MT结合，而鳃中镉蓄积量远远大于MT结合能力．

英文摘要：

To investigate metallothionein （MT） biosynthesis and cadmium accumulation in fresh-water crab Sinopotamon henanense, S. henanense were exposed to 0 （control） ,l mg·L^-1 and 5 rag. L^-1aterburne Cd （as CdCl2·2.5 H2O）. Cd accumulation and MT levels in gills and hepatopancreas were examined with the Cd/Hemoglobin saturation method and flame atomic absorption spectrophotometry. Results showed that mortality in Cd-exposed crabs was minimal （ - 9.2% ）, and no growth effects occurred over the 22d exposure. Cadmium accumulation and MT levels were clearly tissue-speciflc after Cd exposure and revealed some time-dependence and doseldependence. Cadmium levels rose remarkably during the exposure. The gill cadmium levels were much higher than those of the hepatopancreas and reached the highest level at 15. 41 ± 3. 21μg·g^-1. MT concentrations in the gi（ls and hepatopancreas increased significantly after Cd exposure. The hepatopancreas MT levels were much greater than those of the gills and reached the highest level at 97.18 ± 11.83μg·g^-1. Calculation of the Cd-binding potential （CBP） for the gills and hepatopancreas revealed that in the hepatopancreas, MT was theoretically sufficient to bind all the Cd, hut it was not sufficient to sequester all the Cd in the gills.