目的:探索酵母菌在健康大学生肛门直肠的携带情况及菌种分布。方法:随机抽取大理大学健康大学生217名,采集肛门直肠拭子接种于SDA培养基,根据菌落特点、形态染色和生化反应综合鉴定。结果:健康大学生肛门直肠有效标本217份,分离到酵母菌72份,总体携带率为33.2%;其中男生标本98份,分离出酵母菌19株,携带率为19.4%,女生标本119份,分离出酵母菌53株,携带率为44.5%(χ2=15.332,P=0.00)。72株酵母菌中有白假丝酵母菌35株(占48.6%),光滑假丝酵母菌21株(占29.2%),热带假丝酵母菌13株(18.0%),克柔假丝酵母菌2株(占2.8%),其他酵母菌1株(占1.4%)。结论:健康大学生肛门直肠携带多种酵母菌,且以白假丝酵母菌为主。女生肛门直肠酵母菌携带率显著高于男生。
Objective: To explore the carrier status and species distribution of yeast in anus and rectum of healthy college students.Methods: 217 cotton swabs collected randomly from the anus and rectum of healthy college students were inoculated on the modified Sabouraud Dextrose Agar(SDA), Candida species were identified based on the colony characteristics, Gram stain, and biochemical reactions. Results: 72 Candida species were isolated from 217 specimens, the overall carrier rate of yeast in the anus and rectum of healthy college student was 33.2%(72/217); of them, 19 strains were isolated from 98 male specimens, the carrier rate was 19.4%,and 53 strains were isolated from 119 female specimens, the carrier rate was 44.5%(χ2=15.332, P=0.00). Among 72 Candida species,there were 35 strains of C. albicans(48.6%), 21 strains of C. glabrata(29.2%), 13 strains of C. tropicalis(18.1%), 2 strains of C.krusei(2.8%), 1 strain of other Candida species(1.3%). Conclusion: There were various conditioned pathogenic yeasts in the anus and rectum of healthy college students, and C. albicans was the major one. The carrier rate of female students was significantly higher than that of the male students.