中文摘要：

为了检测integrin β1在出生前后大鼠中脑黑质的表达，并探索其在大鼠黑质神经细胞发育过程中的可能作用，本实验采用RT—PCR、Western blot和免疫组织化学染色方法，来测定胚龄（embryonic day，E）E16 d、E18 d、E20 d的健康SD胎鼠及出生后1d（postnatal day1，P1）、P3d、P7d、P14d、P21d的健康仔鼠中脑黑质组织中integrin β1的表达情况。RT—PCR和Western blot结果显示，在检测的各时间点，大鼠中脑黑质integrin β1都有明显表达，且其mRNA和蛋白质水平变化一致。在E16时，integrin β1 mRNA和蛋白质出现高表达，此高表达一直持续到P7时；而至P14时，integrin β1 mRNA和蛋白质表达水平明显降低，且此低水平表达随发育而持续；P21时与P14时相比无明显差异。而免疫组织化学染色结果显示，在各时间点，大鼠中脑黑质一直有大量integrint31阳性细胞存在。在E16～P14各时间点，integrin β1阳性细胞胞体逐渐增大，P21时与P14时相比，阳性细胞胞体大小无明显变化。而单位面积内integrin β1阳性细胞数，在E16-P7各时间点无明显差异，但至P14时，单位面积内integrin β1阳性细胞数则明显降低，且此降低随发育而持续，P21时与P14时相比无明显变化。以上结果表明，在大鼠中脑黑质发育过程中，integrin β1在E16～P7期间持续高表达，而至P14～P21期间，其表达量明显降低。这种表达变化与大鼠发育期间黑质多巴胺（DA）能神经元的自然凋亡时程相一致，提示integrin β1的表达与此期问黑质DA能神经元的凋亡有关。

英文摘要：

In order to define the expression of integrin β1 in mesencephalic substantia nigra of preinatal and postnatal rats and to explore the possible roles of integrin β1 in the developmental course of substantia nigra cells, the RT-PCR, Western blot analysis and immunohistochemistry staining were performed to determine the integrin β1 expression in mesencephalic substantia nigra of healthy Prenatal and postnatal SD rats, aged 16, 18 and 20 embryonic days （El6, E18 and E20） and 1, 3, 7, 14 and21 postnatal days （P1, P3, P7, P14 and P21 ）. The results of RT-PCR and Western blot analysis showed that the mRNA and protein of integrin β1 were obviously expressed in rat substantia nigra at all of the time points of detection in a similar manner. Integrin J31 was highly expressed in substantia nigra from E16 to P7 period ; then the level decreased significantly on P14, and such lowered expression was maintained during the rest of the development, showing no differences between P14 and P21. finmunohistoehemistry staining showed that lots of integrin β1 positive cells were presented in the substantia nigra at all of the time points. The cell bodies increased in size gradually from El6 to P14, and there was no significant difference between P21 and P14. On the other hand, the number of integrin β1 positive cells per area （ cell density） was rather steady, revealed no variation from El6 to P7, but decreased obviously at P14, and this lowered density was maintained during the rest of the development, with no differences between PI4 and P21. The above results indicate that integrin β1 in mesencephalic substantia nigra of rats was highly expressed during the developmental period from E16 to P7 and markedly decreased from P14 to P21. These changes coincide with the timetable of the natural apoptosis of dopaminergic neurons, suggesting that the expression of integrin β1 was associated with the apoptosis of dopaminergic neurons in rat substantia nigra during development.