中文摘要：

目的：研究人B7-H3基因在特异性抗肿瘤免疫中的作用。方法：建人免疫重建荷人口腔鳞癌SCID小鼠嵌合模型,瘤体内注射人B7-H3基因腺病毒表达载体,1周定期检测肿瘤体积,于第4、7周分别尾静脉取血,ELISA法检测人IgG含量;9周后处死模型动物,RT-PCR、免疫荧光显微镜检测肿瘤组织人B7-H3mRNA、蛋白表达;流式细胞仪检测SCID鼠外周血CD4＋、CD8＋T淋巴细胞。结果：第4、7周可在免疫重建小鼠外周血测得人IgG。荧光显微镜观察人B7-H3组小鼠肿瘤组织可见大量绿色荧光,RT-PCR检测到人B7-H3mRNA表达,其余各组均未检测到人B7-H3表达。人B7-H3组小鼠肿瘤体积小于其它组（P〈0.05）,生长明显受到抑制;免疫重建鼠均能检测到人CD4＋、CD8＋T淋巴细胞,人B7-H3组高于其它组。结论：人B7-H3基因在肿瘤组织的表达增强能成功诱导CD4＋、CD8＋T淋巴细胞增殖,产生有效的特异性抗肿瘤免疫应答。

英文摘要：

Objective：To investigate the specific anti-tumor immune function induced by B7-H3 gene in vivo.Methods：Constructed human B7-H3 gene adenovirus expression vector and chimeric mouse model,which carried out human immune reconstitution in SCID mice bearing human oral squamous cell carcinoma.Tumor growth rate was tested every week.Human IgG in blood taking through tail vein was detected by ELISA in the fourth and the seventh week.mRNA and protein expression of B7-H3 gene in tumor tissue were determined by RT-PCR or immunofluorescence microscopy.CD4＋,CD8＋ T lymphocytes in peripheral blood were assessed by flow cytometry.Results：1.human IgG was detected in peripheral blood of immune reconstituted mice.2.B7-H3 mRNA expression and a large number of green fluorescence were detected in tumor tissues of B7-H3 group.3.The tumor volume of B7-H3 group were less than the other group,and the tumour growth was obviously inhibited.4.CD4＋,CD8＋ T lymphocytes were detected in peripheral blood of reconstituted mice,ratio of B7-H3 group was significantly higher than others（P0.05）.Conclusion：In vivo,enhancement of human B7-H3 gene expression in tumor tissues can successfully induce CD4＋,CD8＋ T lymphocytes proliferation,results in an effective specific anti-tumor immune response.