中文摘要：

目的：研究针刺耳“胃”穴及体穴“足三里”治疗实验性胃溃疡过程中，穴区内肥大细胞（mastcells，MC）的分布和功能变化与疗效的相关性。方法：正常成年Wistar大鼠30只随机分为对照组、模型组、电针组，每组10只。后2组首先在胃窦前壁肌层与浆膜层之间注入0．05ml20％冰醋酸制作胃渍疡模型，电针组在造模后连续7d电针（2／15Hz，1mA，30min）胃溃疡大鼠耳“胃”及“足三里”穴。取耳“胃”及“足三里”穴区组织恒冷箱切片，乙酰胆碱酯酶组化染色并用甲苯胺兰复染，显示皮肤真皮层MC的分布、数量和脱颗粒情况；取胃窦前壁含溃疡的胃组织，石蜡包埋，切片，行苏木精-伊红（HE）染色，电镜下观察胃窦前壁黏膜层的形态变化。结果：对3组大鼠的穴区真皮层MC进行对比观察，其中模型组MC总数量均明显多于电针组和对照组（P〈0．01）；模型组MC脱颗粒率明显高于电针组与对照组，电针组细胞脱颗粒率明显多于对照组，3组间差异均非常显著（P〈0．01）。HE染色结果显示：对照组胃壁黏膜面光滑无变化；而模型组胃窦壁黏膜面有很明显的溃疡缺损，并已超过黏膜肌层；电针组胃窦壁黏膜面稍有缺损，尚未达黏膜肌层。结论：耳穴、体穴区组织内MC的总数及脱颗粒率的变化不但可反映胃溃疡的严重程度，还与针刺耳穴及体穴治疗胃溃疡的疗效密切相关。

英文摘要：

Objective To study the interrelation between the therapeutic efficacy of electroacupuncture （EA） and mast cells （MC） in experimental gastric ulcer rats, Methods Thirty Wistar rats were randomly divided into normal control group, model group and EA group, with 10 cases in each. Experimental gastric ulcer model was established by injecting 20% glacial acetic acid （0.05 ml） into the tissue between the muscular layer and the placenta percreta of the gastric anterior wall in the gastric antrum under anesthesia. EA （2/15 Hz, 1 mA） was applied to bilateral ＂Wei＂ （Stomach, an otopoint） and ＂Zusanli＂ （ST 36） for 30 min, once daily for 7 days. The tissues of these two acupoints were sampled to be stained with acetylcholine esterase （ACHE） and toluidine blue （TB） respectively （counterstaining） after section （15 μm）. The gastric ulcer tissue was stained with H＆ E method Results Compared with control group, the number and degranulation ratios of MC in the dermis of ＂Wei＂ -point and ST36 region in model group and EA group increased significantly （P〈0.01）; while in comparison with model group, both the number and the degranulation ratio of MC in EA group decreased considerably （P〈0.01）, H＆ E stained tissues showed smooth gastric mucosal membrane in control group, ulcerative injury involving the muscular layer in model group, and mild injury in EA group. Conclusion EA can suppress the degranulation of MC in ＂Wei＂ -point and ST36 regions and effectively relieve ulcerative injury in gastric ulcer rats. There exists a certain degree of interrelation among the degranulation ratio of MC, gastric ulcerative injury, and the therapeutic efficacy.