中文摘要：

为了确定黄瓜根结线虫侵染早期与取食位点形成相关的WRKy基因，以根结线虫侵染早期的黄瓜根为材料，基于黄瓜全基因组WRKY基因预测，进行RT-PCR扩增。在根结线虫侵染早期的黄瓜根组织中共分离到24个WRKY基因，分别定位于黄瓜的7条染色体上。系统进化分析表明，在分离到的WRKY基因中有17个为第二家族，6个为第一家族，1个属于第三家族。初步确定8个WRKY基因为根结线虫诱导表达基因，推测黄瓜根结线虫侵染早期为涉及多个WRKY基因参与的复杂调控体系，为进一步研究黄瓜根结线虫取食位点发育机制奠定了基础。

英文摘要：

To isolate the WRKY genes from the cucumber root tips infected by root-knot nematode based on the genomic sequence of Cucumis sativus Linn., 24 CsWRKY genes were isolated from the cucumber roots inoculated with Meloidogyne incognita, using RT-PCR method. These genes were located in 7 chromosomes of cucumber respectively. The phylogenetic analysis showed that the 24 CsWRKY genes were classified into three subfamilies, among which 17 genes belonged to group Ⅱ, 6 belonged to group Ⅰ , and 1 belonged to group Ⅲ. We speculated that 8 CsWRKY genes were induced by root-knot nematode. These suggested that feeding-site development of the root-knot nematode was a complex system. It was regulated by some WRKY genes. The isolation and characters of these genes was a basis for the further research on the mechanism of nematode feeding-site development.