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中文摘要：
目的：探讨艾灸防治阿尔茨海默病（Alzheimer Disease,AD）的作用及其机制。方法：大鼠双侧脑室微量注射STZ造模,术后第10天艾灸组灸＂关元＂＂长强＂＂命门＂＂百会＂,同时西药对照组灌胃盐酸多奈哌齐,治疗30 d后用Morris水迷宫测各组大鼠学习记忆能力,再取材用ELISA法、免疫组织化学、Western blot等检测海马区GSK-3β活性及tau蛋白磷酸化位点（Ser396/Thr231）的含量和表达水平。结果：艾灸组与西药对照组较模型组平均游泳距离百分比上升;与假手术组比较,模型组大鼠海马CA1区椎体细胞形态结构异常,阳性染色深,而艾灸组与西药对照组较模型组形态结构完整,阳性染色浅;除假手术组外,GSK-3β活性及磷酸化tau蛋白Ser396、thr231位点相对表达量均升高,且西药对照组和艾灸组低于模型组。结论：艾灸可部分改善AD大鼠学习记忆,其可能通过抑制海马GSK-3β活性,降低p-tau S396、T231的磷酸化水平起到防治AD的作用,且作用效果与西药盐酸多奈哌齐相同。
英文摘要：
Objective To study the effect and mechanism of moxibustion for prevention and treatment of Alzheimer＇s Disease（ AD）. Methods： After making the AD model rats with a pair of lateral ventricle injection of STZ,we observe the rats＇ learning and memory by using Morris water maze,the activity of GSK-3 beta in rats hippocampus by ELISA and Tau protein phosphorylation sites（ Ser396 / Thr231） of content and expression by immunohistochemistry and Western blot technology. Results： The average swimming distance percentage of moxibustion group are more than model group;hippocampus CA1 in model group district vertebral body immune of staining shows cell form exception,positive cell staining deep while in two a treatment group cell form structure more full and positive staining shallower; GSK-3 Beta activity and the phosphate of Tau protein Ser396,thr231 bit points relative expression volume compared： while the model rose to the highest,control group decline below the moxibustion group and the moxibustion below the model. Conclusion： Moxibustion may partly improve learning and memory in AD rats,possibly through inhibition of hippocampal GSK-3β activities,decreased the phosphorylation of phosphorylation levels of Tau protein site Ser396 / Thr231 so as to control AD.
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