中文摘要：

真菌聚酮合酶在代谢中可催化合成多种具有重要生物学活性的次级代谢物，所以真菌聚酮合酶正逐渐成为药学、食品科学和农学等领域的研究热点。综述了近五年来建立的几种分离真菌聚酮合酶基因的方法。这些方法解决了真菌中聚酮合酶基因簇难以分离的问题，为改造和利用真菌聚酮合酶以及发掘真菌聚酮化合物资源提供了强有力的手段。

英文摘要：

Fungal polyketide synthases are responsible for the biosynthesis of secondary metabolites such as pigments, mycotoxins, and they are very important in pharmacology , food science and agriculture. The recent advances in the methods for the isolation and manipulation of multiple classes of polyketide synthase genes from fungi were introduced. It is useful for discovery of novel fungal polyketide synthase gene clusters. These methods can also be useful for revealing the genetic potential of fungi to produce multiple types of bioactive polyketide.