运用Sephadex LH-20、MCI树脂、反相半制备HPLC等多种色谱分离技术,从瑞香科结香属植物结香Edgeworthia chrysantha的内生真菌芬芳镰刀菌Fusarium redolens的大米固体发酵物的乙酸乙酯提取部位分离得到10个化合物,经MS、UV、CD、旋光、IR和NMR等波谱技术分别鉴定为(+)-7S-4-脱氧-9-羟基茎点菌吡喃酮C[(+)-7S-4-deoxy-9-hydroxyphomapyrone C](1)、尿嘧啶(2)、尿苷(3)、2'-去氧尿苷(4)、腺苷(5)、2'-去氧腺苷(6)、cordysinin B(7)、麦角甾醇(8)、麦角甾-5α,8β-环二氧-6,22-二烯-3β-醇(9)和(22E,24S)-3α-羟基-24-甲基胆甾-5,8,22-三烯-7-酮(10)。其中化合物1为新化合物。
A new phomapyrone derivative (1), and 9 known compounds were isolated from the ethyl acetate fraction of solid fermentation of Fusarium redolens, the endophytic fungus from Edgeworthia chrysantha, by using various isolation techniques such as Sephadex LH-20, MCI GEL and Semi pre-HPLC, etc. Their structures were identified by spectroscopic analysis, including MS, UV, CD, specific rotation, IR, 1D and 2D NMR, as (+)-TS-4-deoxy-9-hydroxyphomapyrone C (1), uracil (2), uridine (3), 2'-deoxyuridine (4), adenosine (5), 2'-deoxyadenosine (6), cordysinin B (7), ergosterol (8), ergosta-Sa, 8β-epidioxy-6, 22-dien-3β-ol (9), and (22E, 24S)-3a-hydroxy-24-methylcholesta-5, 8, 22-trien-7-one (10).