中文摘要：

目的采用HPLC法对8批峨参药材的石油醚部位的指纹图谱进行研究。方法采用Shimadzu VP-ODS C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈-0.15%氨水为流动相梯度洗脱,柱温为30℃,检测波长230 nm。结果以13个共有峰为评价指标,8批样品中各共有峰的相对保留时间均小于3%,相似度均大于0.95。结论所用方法操作简便,精密度、稳定性和重复性良好,可为峨参药材的质量控制提供依据。

英文摘要：

OBJECTIVE To establish the fingerprint chromatograms of petroleum ether part form 8 batches of Anthriscus sylvestris by HLPC. METHODS The analysis was performed on a Shimadzu VP- ODS C18column（ 250mm × 4. 6mm,5 μm） with acetonitrile-0. 15% NH3·H2O as mobile phase at a column temperature of 30 ℃,and the detection wavelength was 230 nm. RESULTS 13 peaks were selected as the common fingerprint peaks. The relative retention times of 8 batches of samples were less than 3% and the similarity was more than 0. 95. CONCLUSION The method is easy to operate. The precision,stability and repeatability were qualified. It can be used for the quality control of Anthriscus sylvestris.