中文摘要：

功能的叶绿体产生取决于在质体和原子核之间的基因表示的精确协作并且为植物生长和开发是必要的。在这研究，米饭(Oryza sativa ) 展出了白化体和幼苗致命的显型的异种从 ⁶⁰ 共同照耀的米饭人口。变异的基因作为 Arabidopsis 质体的 ortholog 被识别 transcriptionally 活跃的染色体蛋白质 2 (pTAC2 ) 基因，和变异的种类被指明 osptac2。顺序和抄写分析证明 OspTAC2 编码由 10 pentratricopeptide 重复(PPR ) 组成的通常认为的叶绿体蛋白质领域和 C 终端小傻瓜相关(SMR ) 领域。经由显微镜学的 Cytological 观察证明 OspTAC2 绿荧光灯熔化蛋白质在叶绿体是局部性的。传播电子显微镜学表明 osptac2 异种的叶绿体缺乏组织 thylakoid 膜。所有的抄本层次调查了锐气(编码质体的 RNA 聚合酶) 依赖基因戏剧性地在 osptac2 异种被减少，而抄本 NEP (原子编码聚合酶) 铺平依赖基因被增加。这些结果建议 OspTAC2 在叶绿体开发起一个关键作用并且显示 OspTAC2 基因的分子的功能在米饭和 Arabidopsis 被保存。

英文摘要：

Functional chloroplast generation depends on the precise coordination of gene expression between the plastid and the nucleus and is essential for plant growth and development. In this study, a rice（Oryza sativa） mutant that exhibited albino and seedling-lethal phenotypes was isolated from a60Co-irradiated rice population. The mutant gene was identified as an ortholog of the Arabidopsis plastid transcriptionally active chromosome protein 2（p TAC2） gene, and the mutant strain was designated osptac2. Sequence and transcription analyses showed that Osp TAC2 encodes a putative chloroplast protein consisting of 10 pentratricopeptide repeat（PPR） domains and a C-terminal small Mut S-related（SMR） domain. Cytological observations via microscopy showed that the Osp TAC2-green fluorescent fusion protein is localized in the chloroplasts. Transmission electron microscopy revealed that the chloroplast of the osptac2 mutant lacks an organized thylakoid membrane. The transcript levels of all investigated PEP（plastid-encoded RNA polymerase）-dependent genes were dramatically reduced in the osptac2 mutant, whereas the transcript levels of NEP（nuclear-encoded polymerase）-dependent genes were increased. These results suggest that Osp TAC2 plays a critical role in chloroplast development and indicate that the molecular function of the Osp TAC2 gene is conserved in rice and Arabidopsis.