采用营养液培养的方法,研究了氮、磷、钾元素亏缺对生长发育初期人参根系分泌物组成的影响.结果表明:氮、钾元素亏缺处理的人参根系分泌物组分与对照相当,分别检测到28、29和27个强色谱峰,磷元素缺失处理人参根系分泌物组分相对较少,检测到22种强色谱峰.用化学标准品分别对上述4种处理的根系分泌物中的14、14、11和10个化合物进行了鉴定.与对照相比,氮、磷元素亏缺处理人参根系分泌物有机酸和酚酸类化合物种类增多,磷元素缺失处理人参根系分泌物种类减少.说明人参生长发育初期对氮、钾元素需求比较旺盛,而对磷元素需求较少,氮、钾元素亏缺会促进根对有机酸和酚酸物质的分泌.
By the method of solution culture, the effects of N, P, and K deficiency on the principol components in root exudates of ginseng at its early growth stage were studied. The results showed that in treatments N and K deficiency and control, no significant difference was observed in the principal components of ginseng root exudates, and 28, 29, and 27 principal chromatographic peaks were detected by GC-MS, respectively; while in treatment P deficiency, only 22 principal chromatographic peaks were detected. Furthermore compounds in the root exudates from treatments N, P, and K deficiency and control were identified, respectively. Compared with control, treatments N and K deficiency had more kinds of organic acids and phenolic acids in root exudates, while treatment P deficiency was in adverse, which suggested that at early growth stages, ginseng had more requirement to N and K than P, and N and K deficiency would accelerate the exudation of organic acids and phenolic acids by roots.