中文摘要：

重建的向量 pCANTAB 5 EE 被插入包含了一个 EcoRV 识别序列进 pCANTAB 的 34 搁浅 bpdouble 的 oligonucleotide 获得 5 E。Whitespot 症候群病毒(WSSV ) 染色体 DNA 被 sonication 碎裂主要在 0 的范围孤立碎片。8 ～ 2.0 kb，当时，碎片与 T4 DNA 聚合酶是钝结束的并且克隆 5 EE 进 pCANTAB 的 EcoRV 地点。图书馆的主要 recombinant 克隆是随机的选择 re-combinants 的 5 .Colony PCR 显示出的 3.0 x 10 ~inserts 的尺寸是 0.12 ～ 整个图书馆 recombinant 噬菌体感染了的 1.77 kb.After Escherichi --关口 i HB2151 房间，细胞外并且 periplasmic 摘录在 PVDF 膜上被扔执行点污点，usingpoly同种细胞的老鼠 anti-VP24 浆液， anti-WSV026 浆液， anti-WSV063 浆液， anti-WSV069 浆液， anti-WSV112 浆液， anti-WSV238 浆液， anti-WSV303 浆液和 anti-VP26 浆液结果证明显示图书馆能表示病毒的蛋白质。

英文摘要：

A rebuilt vector pCANTAB 5 EE was obtained by inserting a 34 bp double-stranded oligonucleotide which contained a EcoRV recognition sequence into pCANTAB 5 E. White spot syndrome virus （WSSV） genome DNA was fragmented by sonication to isolate fragments mainly in the range of 0.8 ～2.0 kb, then the fragments were blunt-ended with T4 DNA polymerase and cloned into the EcoRV site of pCANTAB 5 EE. The primary recombinant clone of the library was 3.0 × 10^5.Colony PCR of random selected recombinants showed that the size of the inserts was 0.12 ～ 1.77 kb. After the whole library recombinant phages infected Escherichia coli HB2151 cells, the extracellular and periplasmic extracts were dropped on PVDF membranes to perform dot blot, using polyclonal mouse anti-VP24 serum,anti-WSV026 serum,anti-WSV063 serum,anti-WSV069 serum,anti-WSV112 serum, anti WSV238 serum,anti-WSV303 serum and anti-VP26 serum as the primary antibody, respectively. The results showed that the display library could express the viral proteins.