中文摘要：

背景小岛移植为治疗代表一条理想的治疗学的途径打 1 糖尿病，但是小岛功能和新生可以被氧化应力和另外的侮辱导致的坏死 orapoptosis 影响。Heme oxygenase-1 (HO-1 ) 是在分解代谢的限制 therate 酶他我进 biliverdin，释放的免费的铁和碳一氧化物。是能经由免费激进的清除和 apoptosis 预防由 cytoprotection 改进 grafted 小岛的功能的抗氧化剂酶也被报导了。在现在的学习，我们调查了进有侵入人体气管粘膜的病菌向量的人的小岛的 HO-1 基因的 transduction 是否在在 vitro 有教养的小岛上有 cytoprotective 行动并且为临床的小岛移植讨论基因治疗的这个方法。方法 Cadaveric 胰腺的小岛在 vitro 被孤立并且净化。小岛的 Transduction 效率被与在 2， 5， 10，或 20 的感染(MOI ) 的复合包含提高的绿荧光灯的蛋白质基因(Ad-EGFP ) 的侵入人体气管粘膜的病菌向量感染小岛决定。最新孤立的小岛被划分成三个组：EGFPgroup，有用 MOI=20 的 Ad-EGFP 的小岛 transduced；HO-1 组，有用 MOI=20 包含人的 HO-1 基因的侵入人体气管粘膜的病菌向量的 transduced；并且控制组，嘲笑 transduced 小岛。在房间线的葡萄糖刺激以后的 Insulinrelease 被一个放射性免疫测定工具包决定，刺激索引是计算的。流动 cytometry 被用来由 recombinant 人肿瘤坏死因素在正式就职以后在 HO-1group 并且在控制组检测 apoptotic 房间 -- 为 48 个小时的α(rTNF α) 和酮环己酰亚胺(CHX ) 。结果侵入人体气管粘膜的病菌向量有基因 transduction 的高效率进成年小岛房间。在 transduction andEGFP 在 vitro 在有教养的小岛房间被表示超过四个星期以后，有 Ad-EGFP 的小岛的 Transduction 在 MOI 20，在 MOI，荧光在白天 7 上是很强烈的是很成功的。在控制组的胰岛素版本是(182.36 ± 5 8.96 ) 在由高葡萄糖媒介(16.7 mmol/L ) 的刺激以后的 mIU/L，当从 HO-1 组的胰岛素版本?

英文摘要：

Background Islet transplantation represents an ideal therapeutic approach for treatment of type 1 diabetes but islet function and regeneration may be influenced by necrosis or apoptosis induced by oxidative stress and other insults. Heme oxygenase-1 （HO-1） is the rate-limiting enzyme in the catabolism of heme into biliverdin, releasing free iron and carbon monoxide. It has also been reported to be an antioxidant enzyme which can improve the function of grafted islets by cytoprotection via free radical scavenging and apoptosis prevention. In the present study, we investigated whether transduction of HO-1 genes into human islets with an adenovirus vector has cytoprotective action on islets cultured in vitro and discuss this method of gene therapy for clinical islet transplantation. Methods Cadaveric pancreatic islets were isolated and purified in vitro. Transduction efficiency of islets was determined by infecting islets with adenovirus vector containing the enhanced green fluorescent protein gene （Ad-EGFP） at multiplicities of infection （MOI） of 2, 5, 10, or 20. Newly isolated islets were divided into three groups： EGFP group, islets transduced with Ad-EGFP using MOI=20; HO-1 group, transduced with adenovirus vectors containing the human HO-1 gene using MOI=20; and control group, mock transduced islets. Insulin release after glucose stimulation of the cell lines was determined by a radioimmunoassay kit and the stimulation index was calculated. Flow cytometry was used to detect apoptotic cells in the HO-1 group and in the control group after induction by recombinant human tumor necrosis factor-α （rTNFα） and cycloheximide （CHX） for 48 hours. Results Adenovirus vectors have a high efficiency of gene transduction into adult islet cells. Transduction of islets with the Ad-EGFP was most successful at MOI 20, at which MOI fluorescence was very intense on day 7 after transduction and EGFP was expressed in cultured islet cells for more than four weeks in vitro. The insulin release in the control gro