中文摘要：

采用电喷雾电离离子阱串联质谱检测了1-/2-羟基萘、 2-羟基芴、 2-/3-/4-/9-羟基菲、 6-羟基屈和3-羟基苯并[a]芘等9种不同环数的羟基多环芳烃（OH-PAHs, 2-5环）, 考察了碰撞诱导解离操作参数活化值Q和相对碰撞能量对羟基多环芳烃各单体碎片离子产率的影响. 通过优化活化值Q和相对碰撞能量, 得到了3-羟基苯并[a]芘的碎片离子, 提高了1-羟基萘、 2-羟基芴、 3-/9-羟基菲和6-羟基屈碎片离子的产率, 并发现活化值Q是电喷雾电离离子阱串联质谱检测不同环数PAHs的关键参数.

英文摘要：

Hydroxylated metabolites of polycyclic aromatic hydrocarbons（OH-PAHs） are the mostly widely used biomarker of carcinogenic PAHs to assess health risk caused by exposure to PAHs. Among present strategies used for detecting OH-PAHs, electrospray ionization tandem mass spectrometry（ESI-MS^2） shows advantages in both high sensitivity and selectivity; however, fragment ions cannot be obtained for some OH-PAHs（e.g. 3-OHBaP, 2-OHFlu）. In this work, ESI-MS^2 analysis of nine OH-PAHs（2- to 5-rings） was achieved with ion trap MS^2（IT-MS^2）; by optimizing collision-induced dissociation conditions of activation Q（AQ） and normalized collision energy（NCE）, 3-OHBaP and 2-OHFlu were effectively fragmented meanwhile fragmentation efficiencies of other seven OH-PAHs were also improved. AQ was proven to be the critical parameter for fragmenting OH-PAHs when IT-MS^2 is used.