中文摘要：

目的探讨脂肪组织石蜡切片及冰冻切片方法的改进。方法取脂肪组织迅速固定于10%中性福尔马林中,设置不同的脱水程序制备石蜡切片,HE染色。免疫组化法观察PPARγ和ITLN1的表达。取脂肪组织迅速固定于甲醛钙或10%中性福尔马林固定液中24 h以上,OCT包埋,置于-80℃冰箱至少30 min,设置冰冻切片机箱体温度-20℃,样品头温度-30℃,切片后HE染色。结果用改进方法制备的脂肪组织石蜡切片,切面平整,无皱褶,无裂隙,脂肪细胞结构完整,染色清晰,且对免疫组化结果无影响;冰冻切片的改进提高了切片质量,结构完整,形态好,染色清晰。结论改进的脂肪组织石蜡切片和冰冻切片均可成功应用于不同实验动物,为脂肪组织的研究提供了有力的支撑条件。

英文摘要：

Objective To improve the method of paraffin section and frozen section of adipose tissues. Methods The adipose tissues were collected and quickly placed in 10% neutral formaldehyde. Seetions were prepared by setting different dehydration procedures. The slices were observed under microscope after Hematoxylin-Eosin staining. The expression of PPARγ and ITLN1 in adipose tissues was detected by immunohistochemistry. For frozen sectioning, the adipose tissues were collected and quickly placed in 10% neutral formaldehyde or Calcium formaldehyde, After embedded by OCT, put in -80℃refrigerator for at least 30 minutes. Setting the temperature of frozen section cabinet to -20℃ and the temperature of sample to -30℃. Results Following the improved methods, the slices of adipose tissues were better than before. The structural integrity and consistency of adipose tissues were preserved well, the staining was distinct, and the improved methods had no effect on immunohistochemistry results. The improvement of the frozen sectioning had greatlyimproved the slice quality, the adipose tissues showed complete structure and good morphology. Conclusions The improved methods can be successfully applied to different experimental animals, which provide a powerful condition for the study of adipose tissues.