中文摘要：

目的：探讨人参皂苷Rb1、Rg1、Re对急性髓系白血病细胞株（KG1α）增殖的影响。方法：取对数生长期KG1α细胞,分设人参皂苷Rb1、Rg1、Re组和常规培养组,以MTT比色法检测作用24h、48h、72h时对KGIα细胞增殖抑制作用,并计算Rb1的IC50值,以此浓度为工作浓度,设常规培养组和处理组,台盼蓝计数法观察对KG1α细胞增殖的影响;流式细胞术测定细胞周期分布的变化。结果：MTT、台盼蓝计数显示人参皂苷单体Rb1、Rg1可抑制KG1α细胞增殖,呈浓度依赖性,以Rb1抑制效应最佳,于作用48h抑制率最高。台盼蓝计数显示人参皂苷单体Rb1-120μmol/L作用48h时抑制率达50.22%;流式细胞术结果提示,与对照组比较,Rbl-120μmol/L组G2/M期KG1α细胞比例增加（P〈0.05）。结论：Rb1可抑制KG1α细胞体外增殖,其增殖抑制作用与将KG1α细胞阻滞于G2/M期有关。

英文摘要：

Objective：To investigate the effect of ginsenoside Rb1,Rg1 and Re on proliferation of KG1α cells.Method：After KG1α cells were incubated with 20μmol/L,40μmol/L,60μmol/L,80μmol/L,100μmol/L,120μmol/L,160μmol/L,Rb1 or 5μmol/L,10μmol/L,20μmol/L,40μmol/L ginsenoside Rg1,30μmol/L,60μmol/L,90μmol/L,120μmol/L,150μmol/L ginsenoside Re respectively for 24h,48h and 72h,the conventional culture was performed in blank control group.the the effects of ginsenoside Rb1,Rg1,Re on the proliferation of KG1α cells were examined by MTT assay.IC50 of ginsenoside Rb1（120μmol/L）against KG1α cell for 48 hours was taken as the working concentration in following experiment.trypan blue staining was used to evaluate the ability to inhibit KG1α cell proliferation of ginsenoside Rb1,the effect of the ginsenoside Rb1 on KG1α cell cycle was determined by flow cytometry（FCM）.Result：Ginsenoside Rb1 inhibited KG1α cells proliferation significantly in vitro,compared with Ginsenoside Rg1,Re,which exhibited a dose-dependent manner at range of 20-160 μmol/L,and reached peak at 48h.As determined by FCM analysis,ginsenoside Rb1 arrested the cells in G2/Mphase（P0.05）;in addition,no ＂subdiploid＂ peak appeared.Conclusion：The results suggested that ginsenoside Rb1 may inhibit the proliferation of KG1α cells ex vivo by arresting the cells in G2/M phase.