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病原菌在红壤胶体上的吸附机制研究
  • ISSN号:0564-3929
  • 期刊名称:土壤学报
  • 时间:2013
  • 页码:1-9
  • 分类:S154.3[农业科学—土壤学;农业科学—农业基础科学]
  • 作者机构:[1]华中农业大学农业微生物学国家重点实验室,武汉430070, [2]华中农业大学资源与环境学院,农业部长江中下游耕地保育重点实验室,武汉430070
  • 相关基金:国家自然科学基金项目(41171196,40825002)、国家高技术研究发展计划(2009AA062302)和全国优秀博士学位论文作者专项资金(201066)资助
  • 相关项目:病原菌与土壤颗粒互作的分子机制研究
中文摘要:

研究了pH和KCl离子强度对猪链球菌和大肠杆菌在红壤胶体表面吸附的影响,结合表面物化性质和Derjaguin.Landau—Verwey.Overbeek(DLVO)理论分析互作机制。结果表明,细菌在红壤胶体表面的吸附等温线能较好拟合Freundlich方程(R^2〉0.97),猪链球菌在红壤胶体表面吸附的分配系数(Kf)是大肠杆菌的4.5倍~6.1倍,细菌在去有机质胶体表面吸附的Kf 值为含有机质胶体的2.4倍~3.2倍。比表面积越大或zeta电位绝对值越小,细菌吸附能力越强,吸附态细菌位于距红壤胶体表面90~100nm处的次级小能位置。随着体系pH降低(9.0~4.0)或离子强度增大(1~10mmolL-1),细菌与红壤胶体互作能障降低,细菌吸附量增大,吸附机制符合DLVO理论;而在高离子强度下(50~100mmolL-1),猪链球菌吸附量降低了3.4%~5.6%,表明除DLVO作用力外,非DLVO作用力如空间位阻排斥和疏水作用对吸附也有贡献。

英文摘要:

Effects of soil pH and ionic strength ( KCI ) on adsorption of Streptococcus suis and Escherichia coli on Red soil colloids were studied, and mechanisms of their interactions analyzed from the aspects of surface physicochemical properties of the bacteria and soil colloids and Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. Results show that the adsorption isotherms of bacteria on Red soil colloids fitted well to the Freundlich equation (R2 〉 0.97). The partition co- efficients (Kf) of S. suis adsorption on soil colloids were 4.5 - 6.1 times as high as those of E. coli. The K/values of or- ganic-matter-depleted colloids were 2.4 - 3.2 times as high as those of organic-matter-containing colloids. The bigger the specific surface areas or the lower the absolute zeta potential values, the higher the bacteria adsorption capacity, and the adsorbed bacteria were found at the secondary minimum, 90 - 100 nm apart from the colloids surface. With decreasing so- lution pH (9.0 -4.0) or increasing IS (1 -10 mmol L-1) , the interaction energy barrier between the Red soil colloids and the bacteria decreased and as a result more and more bacteria got adsorbed. This trend was in full agreement with the DLVO theory. The adsorption of S. suis on colloids decreased by 3.4%-5.6% under high IS (50 - 100 mmol L-1), which, besides the DLVO effect, was also ascribed to some non-DLVO effects, like steric repulsion and hydrophobic inter- action.

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期刊信息
  • 《土壤学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国土壤学会
  • 主编:史学正
  • 地址:南京市北京东路71号
  • 邮编:210008
  • 邮箱:actapedo@issas.ac.cn
  • 电话:025-86881237
  • 国际标准刊号:ISSN:0564-3929
  • 国内统一刊号:ISSN:32-1119/P
  • 邮发代号:2-560
  • 获奖情况:
  • 2003年荣获“百种中国杰出学术期刊”称号,2002年荣获“第三届华东地区优秀期刊奖”,2002年荣获“第三届中国科协优秀期刊二等奖”
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:40223