中文摘要：

[目的]阐明左金丸及其拆方对萎缩性胃炎大鼠胃黏膜HSP70基因表达的影响。[方法]建立CAG大鼠模型,并随机分为模型、替普瑞酮、黄连、吴茱萸、左金丸组,设立空白和假手术组,治疗1月后,各组取三只,实时定量PCR法测定各组大鼠胃黏膜中Hsp70mRNA表达量,Western blot法测定Hsp70/Hsp72蛋白表达量,余大鼠HE法观察胃黏膜病理变化。[结果]模型组与其他各组比较,其胃黏膜体积构成比明显下降（P〈0.01）。各治疗组均可以提高胃黏膜体积构成比,但各组间无差异。吴茱萸及左金丸组与模型组相比可以显著增高HSP70mRNA水平（P〈0.05）。在蛋白水平,各治疗组蛋白的表达高于模型组,但与模型组比较差异无统计学意义（P〉0.05）。[结论]中药吴茱萸及左金丸可以改善萎缩性胃炎大鼠胃黏膜的萎缩状态,诱导热休克蛋白70基因表达可能是其治疗萎缩性胃炎的作用机制之一。

英文摘要：

[Objective]To elucidate the effect of Zuojin pill and its separated prescription on expression of the heat shock protein 70（Hsp70）gene in rats with atrophic gastritis.[Methods]To establish the model of chronic atrophic gastritis（CAG）rat which were randomly divided into model group,teprenone group,coptis group,evodia group,and Zuojin pill group,control group and sham operated group.After the onemonth therapeutic phase,three rats were selected from each group,and the expression of Hsp70 mRNA in gastric mucosa was measured by real-time PCR and the expression of Hsp70/72 protein was tested by Western blot method.The remaining rats were observed pathological changes of gastric mucosa by hematoxylin and eosin staining method.[Results]Compared with other groups,the gastric mucosal volume in model group decreased significantly（P〈0.01）.Gastric mucosal volume constituent ratio（GMVR）were increased in all treatment groups,however,there was no difference between the each group.The expression of Hsp70 mRNA in Zuojin pill group and evodia group were higher than the model group（P〈0.05）.The expressions of Hsp70/72 protein in all treatment groups were higher than the model group,while there was no statistical difference（P〉0.05）.[Conclusion]Fructus evodiae and Zuojin pill can improve the state of mucosal atrophy in atrophic gastritis rats.Inducing the Hsp70 gene expression may be one of therapeutic mechanisms for treating atrophic gastritis.