中文摘要：

目的：研究细菌脂多糖（LPS）对小鼠肝脏、肠道羧酸酯酶表达及酶活性的影响。方法：小鼠经腹腔注射5．0mg／kg的LPS，对照组给予生理盐水。注射后24h处死小鼠，取肝脏和肠道组织。用qRT—PCR技术检测小鼠肝脏、肠道组织羧酸酯酶1和2（mCES1和mCES2）mRNA水平；Westernblot技术检测小鼠肝脏、肠道组织mCES1和mCES2蛋白表达水平。用分光光度计检测小鼠肝脏、肠道组织羧酸酯酶总活性。结果：LPS显著降低小鼠肝脏、肠道组织羧酸酯酶1和2的mRNA水平（P〈0．05），同时LPS也显著降低小鼠肝脏、肠道组织羧酸酯酶的蛋白表达水平及酶活性（P〈0．05）。结论：LPS造成的炎症状态可显著降低小鼠肝脏、肠道组织羧酸酯酶的表达及酶活性。

英文摘要：

Objective： To investigate the effects of lipopolysaccharide （LPS） on the expressions of carboxylesterasel and 2 in mouse liver and intestine. Methods： All mice except controls received an intraperitoneal injection ofLPS 5.0mg / kg. The mice were sacrificed at 24h aRer LPS treatment. Liver and Intestine carboxylesterasel and 2 mRNA were determined by real-time PCR. Carboxylesterase activities was measured by the para-nitrophenylacetate reagent. Liver and Intestine carboxylesterasel and 2 protein levels were determined by Western Blot. Results： LPS not only inhibited the expressions of liver carboxylesterasel and 2, but also down-regulated the levels of carboxylesterase 1 and 2 of intestine. Furthermore, LPS repressed the carboxylesterase catalytic activity in mice livers and intestines. Conclusions： LPS suppresses the expressions of carboxylesterase 1 and 2 in mouse liver and intestine.