中文摘要：

目的：探讨NOX1对TNF-α诱导的A549细胞凋亡的影响。方法：将A549细胞分为3组,空白组、TNF-α组和TNF-α＋NOX1 siRNA组。TNF-α＋NOX1 siRNA组细胞采用瞬时转染技术转染特异性NOX1 siRNA,转染12 h后用含TNF-α（10 μg/L）的培养基继续培养; 空白组和TNF-α组细胞分别用培养基和含TNF-α的培养基培养。48 h后,采用Annexin V-FITC和PI双染法检测细胞凋亡率,DCFH荧光标记法检测细胞中ROS的表达量,Western blot法检测NOX1及p-JNK蛋白的表达水平。结果：与空白组相比,TNF-α组细胞凋亡率和ROS表达量升高（P＜0.05）,NOX1和p-JNK蛋白表达水平升高（P＜0.05）; 与TNF-α组比较,TNF-α＋NOX1 siRNA组细胞凋亡率和ROS表达量降低（P＜0.05）,细胞中NOX1和p-JNK蛋白表达水平也降低（P＜0.05）。结论：NOX1可能通过增加ROS的表达,进而激活JNK/MAPK信号通路,引起A549细胞的凋亡。

英文摘要：

Aim： To investigate the effect of NOX1 on apoptosis of A549 cells induced by TNF-α.Methods： A549 cells were allocated into 3 groups：blank control group,TNF-α group and TNF-α＋NOX1 siRNA group. Cells in TNF-α＋NOX1 siRNA group were transfected with NOX1 siRNA through the transient transfection technology for 12 h,then cultured with TNF-α（10 μg/L）for 48 h. Cells in blank control group and TNF-α group were only cultured with medium and TNF-α（10 μg/L）for 48 h,respectively.After culture, the apoptosis rate was detected by Annexin V-FITC and PI staining,the ROS level in cells was detected by DCFH fluorescent probe method, and the expressions of NOX1 and p-JNK protein were detected through Western blot.Results： Compared with blank control group,the apoptosis rate,ROS level and the expressions of NOX1 and p-JNK were increased in TNF-α group（P〈0.05）; while compared with TNF-α group, the apoptosis rate,ROS level and the expressions of NOX1 and p-JNK were decreased in TNF-α＋NOX1 siRNA group（P〈0.05）.Conclusion： NOX1 could increase ROS level, then active JNK/MAPK signal pathway, and induce A549 cell apoptosis.