依托中国科学院黄土高原长武农业生态试验站中的长期定位试验(始于1984年),利用湿筛法获得不同粒径的团聚体,研究了长期施肥对不同粒级水稳性团聚体中有机碳分布的影响。试验涉及化肥和轮作培肥两个长期试验的9个处理。化肥试验:同一施磷基础上的5个施氮水平(N0、N45、N90、N135、N180);轮作培肥试验:不施肥(CK)、化肥(NP)、有机肥(M)、化肥有机肥配施(NPM)。结果表明:长期施肥显著影响土壤水稳性团聚体含量(p〈0.05),提高了〉2mm粒级水稳性团聚体含量,降低了〈0.25mm水稳性团聚体含量。施氮(N45、N90、N135、N180)处理主要提高了〉5mm、0.5~0.25mm水稳性团聚体中有机碳的含量,与N0相比,提高量为44.3%~73.3%;有机肥(M、NPM)处理对各粒级团聚体中有机碳的含量均有提高,与CK相比,提高量为40.7%~92.2%,其中〉5mm团聚体中有机碳含量分别提高了66.7%和92.2%。低氮(N0、N45、N90)处理、不施肥(CK)处理的1~0.5mm团聚体中的有机碳对土壤有机碳的贡献率最大,占13.7%~23.7%;高氮(N135、N180)处理和氮磷配施(NP)处理、有机肥(M、NPM)处理的〉5mm团聚体中的有机碳对土壤有机碳的贡献率最大,占17.3%~24.9%。土壤有机碳与〉5mm、5~2mm团聚体含量呈显著正相关关系,与〈0.25mm团聚体含量呈极显著负相关。
Various particle-size fractions of soil aggregates were obtained with the wet-screening method from the soil of a long-term stationary fertilization experiment(since 1984) field of the Changwu State Key Loess Plateau Agro-Ecological Experimental Station of the Chinese Academy of Sciences,Northwest China,for analysis of effect of long term fertilization on soil organic carbon distribution in water-stable aggregates of different particle-size fractions.The experiment had five N treatments(N0,N45,N90,N135,and N180),which were all the same in P application rate,and four fertilization treatments,that is,CK(unfertilized),NP(nitrogen and phosphorus),M(farmyard manure),and NPM(nitrogen and phosphorus plus farmyard manure).Results show that long-term fertilization significantly affected water-stable aggregates distribution(p0.05),increasing the content of macro aggregates(2 mm) and decreasing that of micro aggregates(0.25 mm).Nitrogen fertilization significantly increased soil organic carbon(SOC) content in the 0.5~0.25 mm and5mm water-stable aggregates by 44.3%~73.3%,compared to that in Treatment N0.Moreover,Treatments M and NPM increased SOC content in aggregates of all particle sizes by 40.7%~92.2% over CK,and in the 5 mm aggregates by 66.7% and 92.2%,respectively.Treatments N0,N45 N90and CK contributed the most to the soil organic carbon content in the 1~0.5 mm water-stable aggregates,accounting for 13.7%~23.7%.Treatments N135,N180,NP,M and NPM contributed the most to the SOC content in the5 mm aggregates,accounting for 17.3%~24.9%.SOC was positively related to the content of the5 mm and 5~2 mm water-stable aggregates,but negatively related to the content of the0.25 mm water-stable aggregates.