中文摘要：

以初始体重（3.17±0.01）g的皱纹盘鲍（Haliotis discus hannai）为研究对象,在其饲料中分别添加0、0.7和2.1g/kg的α-硫辛酸配制成3种半精制饲料,在水体Cu含量为0.02mg/L的静水养殖系统中饲养60d,探讨饲料硫辛酸在水体Cu胁迫条件下对皱纹盘鲍的保护作用。结果表明,皱纹盘鲍的特定生长率（SGR）随着饲料中硫辛酸的增加而升高,但与对照组无显著差异。0.7和2.1g/kg硫辛酸处理组皱纹盘鲍的血清、外套膜、鳃和肝胰脏中Cu含量显著低于对照组,2.1g/kg硫辛酸处理组的肌肉中Cu含量显著低于对照组,但是0.7g/kg硫辛酸处理组肌肉Cu含量与对照组无显著差异。硫辛酸的各添加水平显著升高Cu胁迫下皱纹盘鲍的肝胰脏超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽硫转移酶（GST）的活力及还原型谷胱甘肽（GSH）的含量。0.7和2.1g/kg硫辛酸处理组的肝胰脏丙二醛（MDA）的含量显著低于对照组。2.1g/kg硫辛酸处理组肝胰脏蛋白羰基的含量和DNA断裂损伤显著低于对照组和0.7g/kg处理组。由此可见,饲料中的硫辛酸可以显著升高Cu胁迫下的皱纹盘鲍肝胰脏抗氧化水平,显著降低皱纹盘鲍组织Cu的沉积量,并在一定程度上减轻了肝胰脏蛋白质、DNA损伤和脂质过氧化。

英文摘要：

A feeding trial was conducted to assess the protective effect of dietaryα-lipoic acid（LA）on juvenile abalone Haliotisdiscus hannai Ino against the toxicity of waterborne copper（Cu）.Aabalone（initial weight（3.17±0.01）g were fed with the diets supplemented with 0、0.7and 2.1g/kg of LA each for 60 days under waterborne Cu exposure（0.02mg/L）in a static water system.Results showed that the specific growth rate（SGR）of abalone increased with the dietary LA contents.However,no significant difference was found among diets.Compared with that of control,Cu concentration in serum,mantle,gill and hepatopancreas significantly decreased in abalone fed with 0.7and 2.1g/kg LA supplemented diets.The abalone fed with 2.1g/kg LA supplemented diets had significantly lower Cu concentration in muscle than that in the control.Such decrease was not observed in abalone fed with 0.7g/kg LA supplemented diets.Activities of superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GPx）,glutathione S-transferase（GST）and the content of glutathione（GSH）in hepatopancreas significantly increased with the dietary LA contents.Compared with that of control,the malondiadehyde（MDA）content in abalone fed with 0.7and 2.1g/kg LA supplemented diets significantly decreased.Protein carbonyl content and DNA strand-breaks in abalone fed with 2.1g/kg LA supplemented diets significantly decreased.The present findings indicated that dietary LA increased the anti-oxidative capacity of abalone and decreased the Cu concentration in tissues.To some extent,dietary LA decreased protein carbonyl content,DNA strandbreaks and the level of lipid peroxidation in hepatopancreas of abalone.