中文摘要：

应用改进的TRAP-银染法检测了鸡胚成纤维细胞（CEF）和马立克氏淋巴瘤细胞系（MDCC-MSB1）的端粒酶活性,并应用荧光相对定量RT-PCR法,检测了这2种细胞中的端粒酶基因：端粒酶反转录酶（Telomerase reverse transcriptase,chTERT）和端粒酶RNA（Telomerase RNA,chTER）的相对表达量,以探讨端粒酶活性与基因表达的相关性。结果显示,MDCC-MSB1细胞的相对端粒酶活力是CEF细胞的100倍;实时荧光定量检测,MDCC-MSB1细胞中chTERT的mRNA表达水平高于chTER基因,其中chTERT基因的表达量为CEF的215倍,而chTER基因的表达量只有CEF的10倍。研究表明,chTER基因在CEF和MDCC-MSB1的表达差异不显著,而chTERT基因的mRNA表达水平在MDCC-MSB1细胞中却显著增加,说明chTERT是端粒酶活性的主要限速步骤之一,也可能是导致马立克氏病淋巴瘤的重要因素。

英文摘要：

The updated TRAP Argentum-dying method was used to test the activity of the telomerase in chicken embryo fibroblasts （CEF） and MDCC-MSBI, and employing real-time quantitative polymerase chain reaction, through the Taqman quantitative testing technique to test the expression of the two telomerase genes in the cell, telomerase reverse transcriptase（chTERT） and telomerase RNA（chTER） .The result showed that the activity of telomerase in MDCC-MSB1 was much higher than in CEF; In real-time quantitative polymerase chain reaction, the expression of chTER in the highly-active MDCC-MSB1 was 10 times higher than in CEF while the expression of mRNA in chTERT increases 215 times from those in CEF.The fact of the little difference with chTER expression between CEF and MDCC-MSB1, and drastic rise with the mRNA in chTERT of MDCC-MSB1 indicated that this mRNA can be a checking dement for activity of telomerase and it can also be an important factor leading to Marek＇ s disease lymphadenoma.