利用简单重复序列间扩增(ISSR)分子标记,分析了舟山群岛3个岛屿4个滨柃种群的遗传结构,比较了分布区域不同的3个物种滨柃、全缘冬青与红楠遗传变异的差异。滨柃种群平均多态性百分比(Lpp)为37.95%,有效等位基因数(Ne)为1.223,Nei's基因多样性(H)为0.132,Shannon多态性信息指数(I)为0.200,均低于全缘冬青与红楠的遗传多样性水平。滨柃种群间遗传分化指数(Gst)为0.202,远低于全缘冬青(0.316)及红楠(0.311)。个体间UPGMA聚类表明,岛屿隔离没有完全阻隔滨柃在不同岛屿间的交流,植物种子随海水漂移降低了其种群间遗传分化水平,而全缘冬青与红楠其种群间交流则受到岛屿的严重隔离。
we used inter-simple sequence repeat (ISSR) markers to investigate the genetic structure of Eurya emarginated Makine for four populations collected from three islands in the Zhoushan ArChipelago, and the result was then used for genetic diversity comparison between Eurya emarginated Makine, Ilex integra and Machilus thunbergii. The average values of percentage of polymorphic loci is 37.95%, effective number of alleles (Ne=1.223), Nei's genetic diversity (H=0.132) and Shannon's information index (I=0.200) indicated that the Eurya population had lower genetic variations than both the Ilex integra and Machilus thunbergii species. The genetic differentiation for populations of Eurya emarginated Makine (Gst=0.202) is lower than those of Ilex integra and Machilus thunbergii. UPGMA cluster for individuals revealed that the isolation between the islands could not completely obstruct the genetic exchanges between different populations of Eurya emarginated Makine, and the excursion of plant seeds floating depressed the value of genetic differentiation, while the exchanges of Ilex integra and Machilus thunbergii populations were greatly isolated by the islands.