中文摘要：

建立了人体尿液中蝶呤-6-羧酸的高效液相色谱-紫外分析新方法。运用Lichrospher C18柱（250×4．6mm，5μm），甲醇-水（70：30，V／V）为流动相，流速为0．4mL／min，可较好地将尿液中蝶呤-6-羧酸与其它共存干扰物质分离。在355nm检测波长下，蝶呤-6-羧酸在0．19～4．8μg／mL范围内与色谱峰面积呈良好线性关系，相关系数为0．9999，方法检出限为0．015μg／mL。尿液经0．45μm滤膜过滤后，可直接进样分析，方法简便，应用于癌症病人和健康人尿样中蝶呤-6-羧酸测定，结果较好。方法的加标回收率为94．6％～100．2％，相对标准偏差为0．81％～5．07％。

英文摘要：

A new method based on high-performance liquid chromatography-ultraviolet detection was established for the determination of pterin-6-carboxylic acid in human urine. After filtrated, urine samples were directly separated on a Lichrospher C18 column by using methanol-water （70： 30 ,V/V） as mobile phase, at the flow rate of 0.4 mL/min and the detection wavelength of 355 nm. The proposed method has successfully applied to the determination of the content of pterin-6-carboxylic acid in both healthy human urine and the cancer patients ＇ urine samples. The intensity of absorbance was proportional to the concentration of pterin-6-carboxylic acid over the range of 0.19-4.8 μg/mL, with the linear correlation coefficient of 0. 9999. The average recoveries were between 94.6% and 100.2%, and the relative standard deviation was less than 5.07 %.