中文摘要：

将pib基因上游5．7kb区段取代pCAMBIA1301中gus基因上游的35S启动子，构建了pib拟启动区-GUS＋35S-hpt植物表达载体pNAR604。经农杆菌介导转化水稻成熟胚愈伤，获得了转基因抗潮霉素愈伤和36株转基因水稻植株。转基因抗性愈伤和转基因植株根的组织化学GUS活性检测表明，光照培养下的抗性愈伤和转基因植株根不能使X—gluc显色，而暗处理24h后的抗性愈伤和定植后转基因植株的根能使X-gluc显色。转基因植株GUS荧光定量分析结果表明，GUS表达具有器官特异性，黑暗处理前根的GUS活性最高、茎次之，分别是叶片的7倍和3倍，叶片中仅有痕量本底。24h黑暗处理后根、茎、叶中GUS活性都有增加，且叶片中的增加比例最大，其活性仅次于根。5mmol／L水杨酸和0．3mol／L NaCl叶面喷施转基因植株24h后叶片中GUS活性分别为处理前的2．7和3．6倍。初步确定pib拟启动区是一个诱导型启动子。黑暗、水杨酸和NaCl能诱导该启动子启动活性。

英文摘要：

A 5.7 kb putative promoter region of pib gene was isolated from the pib genomic clone and substituted for the 35S promoter upstream of gus gene in plasmid pCAMBIA1301 to construct a new plant expression vector pNAR604 （putative pib promoter-GUS ＋ 35S-hpt）. From Agrobacterium-mediated transformation and hygromycin selective cul- ture in vitro, hygromycin resistant calli and 36 transgenic rice （Oryza saliva L. ） plants were obtained. Histochemical assays of GUS activity showed that no expression was observed in the resistant calli and roots from transgenic rice if cultured under light, but after 24 h dark treatment there was strong GUS staining. Fluorimetric quantitative analysis indicated that GUS expression was organ-specific in transgenic rice. Without the dark treatment, GUS activity in roots and stems were about 7 and 3 times higher than in leaves in which GUS activity was only trace detected. After 24 h dark treatment, GUS activity in roots, stems and leaves of transgenic plants were all promoted and the largest increase was observed in leaves. Twenty-four huors after spraying with 5 mmol/L SA （Salicylic Acid） or 0.3 mol/L NaCI, GUS activity in leaves of the transgenic plants was 2.7 or 3.6 times respectively higher than untreated control. It was con-firmed that an inductive promoter was involved in this 5.7 kb upstream region of pib gene, and dark, SA and NaCI treatments were inductive factors for pib promoter.