中文摘要：

通过保留多溴联苯苯环和在苯环上引入氨基取代基和羧基取代基，得到两种多溴联苯半抗原．然后用重氮化法和活化酯法，把半抗原分别与牛血清蛋白偶联，合成了两种人工免疫原，抗原结合比为1：17和1：19．以此免疫原免疫新西兰大白兔，双向琼脂扩散方法测定血清效价可达1：32—1：64．

英文摘要：

In order to establish a new method for the PBBs detection by immuno-PCR, two sorts polybrominated biphenyls hapten was synthesized by introducing amino group and carboxyl group as a substituent at the aromatic ring while retaining the biphenyls ring. The hapten was conjugated to BSA via amino diazotization linkage and by the NHS ester method to get immunogen. The conjugation products was identified by ultraviolet spectrophotometry. The results showed that the molar incorporation ratio reached 1：17 and 1： 19. New Zeal- and rabbits were used to injected immunogens to validate the haptens. The antiserum exhibited relatively high antibody titres （1： 32-1： 64） in double agar diffusion.