中文摘要：

目的：采用HPLC-ICP-MS对藏药仁青芒觉中可溶性砷的形态进行研究。方法：采用Agilent G3154A阴离子交换柱,以0.2 mmol.L^-1 EDTA和2 mmol.L^-1磷酸二氢钠的溶液（pH 6.0）为流动相,流速1.0 mL.min^-1,测定了仁青芒觉中砷的形态。前处理选择微波萃取,比较了1%盐酸、1%硝酸、2%醋酸、50%甲醇不同萃取剂的提取效率。结果：仁青芒觉在不同提取液中砷的形态主要以As（Ⅲ）和As（V）的形式存在,且1%盐酸、1%硝酸的提取效率较高。As（Ⅲ）和As（V）检出限（S/N=3）分别为0.39 ng.g-1和0.72 ng.g-1,样品加标平均萃取回收率（n=3）分别为95.6%和85.3%,精密度试验的RSD（n=8）分别为2.0%和3.2%。结论：藏药仁青芒觉中可溶性砷形态的研究,为藏药中砷的毒理学研究提供了依据。

英文摘要：

Objective：To study the speciation of solvable arsenic in Renqing Mangjue.Methods：High performance liquid chromatography with Agilent G3154A anion-exchange column coupled with inductively coupled plasma-mass spectrometry were adopted.The optimized mobile phase composition was 2.0 mmol·L^-1 sodium dihydrogen phosphate,0.2 mmol·L^-1 EDTA at pH 6.0.Micro wave-assisted extraction was chosed as pre-treatment method,the extract efficiencies of different extractants（1% hydrochloric acid,1% nitric acid,2% acetic acid,50% methanol） were compared.Results：The result indicated that the main speciation of As（Ⅲ） and As（V） was presented in extraction solutions of Renqing Mangjue and 1% hydrochloric acid and 1% nitric acid had high extract efficiency.The limits of detection（S/N=3） of As（Ⅲ） and As（V） were 0.39 ng·g-1 and 0.72 ng·g-1 respectively;Average spiked recoveries（n=3） were 95.6% and 85.3% respectively;RSDs of the precision test（n=8）were 2.0% and 3.2%.Conclusion：Speciation analysis of solvable arsenic in Renqing Mangjue by HPLC-ICP-MS has offered a reference for toxicology of arsenic in Tibetan medicine.