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中文摘要：
为了研究盐度10条件下中华鲟幼鱼鳃丝Na＋,K＋-ATPase（NKA）α亚基的分子调节机制,采用克隆方法得到中华鲟幼鱼鳃丝NKAα亚基部分基因序列,在鲟鱼转移后3,6,12,24,48,72和96 h检测实验组（淡水-盐水）和对照组（淡水-淡水）中华鲟幼鱼鳃丝NKAα亚基的mRNA表达水平、鳃丝NKA活性、血清Na＋、Cl-的浓度和渗透压。结果显示,在转移后0～12 h,实验组中的mRNA表达量与酶活性为适应盐度变化而显著增加（P〈0.05）,离子浓度和渗透压均显著上升（P〈0.05）;在转移后12～24 h,mRNA表达和酶活性开始降低到一个较低值,但仍然显著高于对照组的值（P〈0.05）,离子﹑渗透压的变化与酶活性及mRNA变化趋势一致,这表明中华鲟鳃丝NKA在应对盐度变化时通过改变mRNA表达量来增加酶活性,高活性NKA调节血清离子和渗透压平衡。
英文摘要：
In order to explore the molecular regulation mechanism of Na ＋ , K ＋ -ATPase （NKA） α-subunit in gills of juvenile Chinese sturgeon at salinity 10, partial NKA α-subunit gene sequence was cloned, and the abundance of α-subunit mRNA,NKA activity, ion concentration and osmolality were examined at 3,6,12, 24,48,72,96 hours post-transfer. Results showed that：in experimental group（ fresh water-salinity water ,FW- SW） ,the expression of NKA α-subunit mRNA and NKA activity increased significantly （ P 〈 0.05 ） during the adaptation phase （0 -12 h）, and the concentration of Na ＋ , C1-and osmolality rose to a maximum level at 12 h post-transfer. In regulatory phase（ 12 -24 h）,the amounts of NKA α-subunit mRNA and NKA activity fell to a relatively lower level which was still higher than that of fish in control group （P 〈 0.05 ）. The change both in ion concentration and osmolality were in accordance with the change of NKA and mRNA expression. In conclusion, gill Na ＋ , K ＋ -ATPase in Chinese sturgeon plays an important role in osmolality regulation by changing α-subunit mRNA expression. The different α-subunit mRNA expression can lead to the variation of enzyme activity which can further regulate the ion concentration and osmolality.
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