中文摘要：

为研究Sch9激酶的生理功能,本文首先通过同步细胞周期的方法 发现Sch9的PDK1位点的磷酸化随细胞周期变化.接下来在酵母中过表达Cdc34显示Cdc34的组成性表达可以进一步延长s ch9△突变体的寿命并且增强其对氧化胁迫及热胁迫的抗性.我们的研究结果首次表明Cdc34作为Sch9的底物参与细胞胁迫应答和衰老的调控.

英文摘要：

With the purpose of examining the physiological function of Sch9 kinase, the variable phos-phorylation statuses of PDK1 site of Sch9 during different cell phases were monitored using synchronizedyeast. Additionally, Overexpressing Cdc34 in Sch9 deficient yeast cells was able to further lengthen thelongevity and increased cellular resistance to oxidative and thermal stress. These data imply for the firsttime that Cdc34 participated in longevity regulation and stress response as a substrate of Sch9.