中文摘要：

目的：观察不同氧浓度环境下不同时间处理对大鼠大脑皮层细胞表达IL-1β、IL-6和TNF-αmRNA的影响。方法：原代培养新生SD大鼠大脑皮层细胞，设1％、4％两个低氧浓度和3、6h两个低氧处理时间，处理结束后收集各组细胞，采用实时荧光定量PCR（RT—QPCR）方法检测各组细胞表达IL-1β、IL-6和TNF-αmRNA情况。结果：与相应的常氧对照组相比，1％和4％氧浓度环境处理细胞3h和6h时，IL-1β和TNF-αmRNA表达量均无明显变化（P〉0．05）；处理细胞3h时，IL-6mRNA表达量均无明显变化（P〉0．05），但有上升的趋势，处理细胞6h时IL-6mRNA表达量均显著升高（P〈0．05）。结论：1％和4％氧浓度环境虽均对大脑皮层细胞表达IL-1β和TNF-αmRNA无影响，但可诱导细胞表达IL-6mRNA，且具有时间依赖性。

英文摘要：

Objective： To investigate the effects of different time treatment on levels of IL-1β , IL-6 and TNF-α mRNA expressed by cerebral cortex cells in rat under different oxygen concentration. Methods： Primary cultured cerebral cortex cells were subjected to hypoxia （ 1% and 4% ） for 3 hours and 6 hours. The production of IL-1β , IL-6 and TNF-α mRNA were analyzed by RT-QPCR. Results： Compared with matched normoxic controls, the production of IL-1β and TNF-α mRNA had no significant difference when cells were subjected to hypoxia （1% and 4% ） for 3 hours or 6 hours; and the production of IL-6 mRNA had no significant difference when cells were subjected to hypoxia （1% and 4% ） for 3h, but had an increased trend; while the levels of IL-6 mRNA were significantly increased when subjected to hypoxia （ 1% and 4% ） for 6 hours. Conclusion： The stimulation of hypoxia （ 1% and 4% ） on the production of IL-6mRNA had a time-dependent, but hypoxia had no effect on the production of IL-1β and TNF-α mRNA.