中文摘要：

目的探讨槐定碱不同给药方式对内毒素诱导的小鼠RAW264.7巨噬细胞表达CD14、p38 MAPK及i NOS的影响及意义。方法采用LPS诱导的RAW264.7巨噬细胞建立细胞炎症反应模型;实验细胞分为4组（n=6）：空白对照组、LPS组、槐定碱预处理组、槐定碱预混合组;分别利用反转录聚合酶链反应（RT-PCR）和Western blot技术检测RAW264.7巨噬细胞CD14、p38、i NOS m RNA表达量及蛋白表达量。结果槐定碱2种给药方式（预处理及预混合）均可显著下调LPS诱导的RAW264.7巨噬细胞p38、i NOS m RNA及CD14、p-p38、i NOS蛋白表达;槐定碱与LPS预混合可下调LPS诱导的RAW264.7巨噬细胞CD14 m RNA表达,而槐定碱预处理对CD14 m RNA表达无明显影响。结论槐定碱可能通过调控CD14、p38、i NOS表达与活化发挥抗内毒素效应。

英文摘要：

To investigate the roles of sophoridine（SRI） in inflammation induced by lipopolysaccharide（LPS） in two different modes of administration,LPS-activated macrophage cells（RAW264.7） was employed as an inflammation model. RAW264.7 cells were divided into 4 groups（n=6）： control group,LPS group,SRI pretreating group,SRI premixing group. The m RNA and protein expressions of CD14,p38,i NOS were measured by using reverse transcription polymerase chain reaction（RT-PCR） and Western blotting,respectively. Data showed that compared with LPS group,the expressions of CD14,p-p38 and i NOS protein and p38,i NOS m RNA were significantly decreased in SRI pretreating group and SRI premixing group（P〈0.01）,which indicated that SRI can inhibit the expression of CD14,p-p38 and i NOS protein and p38,i NOS m RNA induced by LPS in RAW264.7 cells.Furthermore,compared with SRI pretreating group,the expression of CD14 m RNA was significantly decreased in SRI premixing group（P〈 0.01）,which indicated that SRI premixing group can abolish expression of CD14 m RNA.Taken together,SRI can play anti-endotoxin role in inflammation by decreasing expressions of CD14,p-p38,i NOS protein and p38,i NOS m RNA in TLR4 pathway.