中文摘要：

为了识别基因，把过渡房间癌与人的膀胱的词法显型联系了，我们使用了减少性的杂交( SSH )建立了创造二的一个减少性的 cDNA 图书馆的抑制房间线， BLZ-211 和 BLS-211 ，与膀胱的过渡房间癌源于一个病人，然后表示了基因为差别屏蔽。实时反向的抄写聚合酶链反应被用来进一步证实选择差别表示了基因。前面、反向的减少性的 cDNA 图书馆产出 168 和 305 通常认为的克隆，并且在他们之中，超过 90% 包含了 inserts。在微分屏蔽以后， 36 个不同抄本从一个前面、反向的减法图书馆的 64 cDNA 克隆被获得。在他们之中， 17 是例如，由相同知道基因被识别 Vimentin， Keratin7， DDH 和 UCH-L1。留下 19 是未知的表示了基因，并且作为由 GenBank dbEST 数据库的新表示顺序标签镇定。他们的功能将进一步被学习。因此， SSH 看起来是为鉴别差别表示了在房间线或克隆之间的基因的一种有用技术。我们的结果由 SSH 揭示了也建议在细胞骨架蛋白质的基因表示的差别可能在 BLZ-211 和 BLS-211 房间贡献不同形态学。

英文摘要：

To identify genes associated with morphological phenotypes of human bladder transitional cell carcinoma, we used suppression subtractive hybridization （SSH） to create a subtractive cDNA library of two established cell lines, BLZ-211 and BLS-211, derived from a patient with transitional cell carcinoma of the bladder, then to screen for differentially expressed genes. Real-time reverse transcription-polymerase chain reaction was used to further confirm the selected differentially expressed genes. Forward and reverse subtractive cDNA libraries yielded 168 and 305 putative clones, and among them more than 90% contained the inserts. After differential screening, 36 different transcripts were obtained from 64 cDNA clones of a forward and reverse subtraction library. Among them, 17 were identified as known genes by homology, for example, Vimentin, Keratin 7, DDH and UCH-L1. The remaining 19 were unknown expressed genes, and were collected as new expressed sequence tags by the GenBank dbEST database with the accession numbers DR008207, DR010178, DR 159652-DR159660, DY230447-DY230448, and DY505708-DY505713. Their function will be studied further. Thus, SSH appears to be a useful technique for identifying differentially expressed genes between cell lines or clones. Our results, as revealed by SSH, also suggest that differences in gene expression of cytoskeletal proteins might contribute to the different morphologies in BLZ-211 and BLS-211 cells.