目的:提取分离海柳的挥发性成分并鉴定。方法:采用二氧化碳超临界萃取出海柳挥发性成分,再经气质联用分析,结合保留指数计算,鉴定其成分。结果:通过气质联用分析,共鉴定出15种化学成分,包括磷酸三乙酯、丁羟甲苯、雪松醇、棕榈酸、角鲨烯、胆甾醇等。这些成分多具有生物活性。结论:首次揭示海柳的挥发性成分,海柳所具有的功效可能与其含有的生物活性成分有关。
OBJECTIVE:To investigate the volatile constituents of black coral(BC).METHODS:The volatile constituents of BC were extracted by supercritical fluid extraction with carbon dioxide(CO_2-SFE),separated and identified by gas chromatography-mass spectrometry(GC-MS)combined with retention index.RESULTS:In total,15 components were reliably identified in BC and found to be biologically active.These included triethyl phosphate, butylated hydroxytoluene,cedrol,n-hexadecanoic acid,squalene and cholesterol.CONCLUSION:It is the first time that volatile constituents of BC were presented.The functions of BC are related to the active chemical compositions.