通过实时调控SBR反应器内的游离氨浓度的控制策略,实现以亚硝化作用和厌氧氨氧化作用协同的单级自养生物脱氮工艺.实验分成亚硝酸菌富集和厌氧氨氧化菌混合接种2个阶段,SBR内的温度始终保持在(31±2)℃.亚硝酸菌富集阶段,pH值稳定在7.8左右,通过调节进水氨氮浓度(56~446mg·L^-1)实现FA浓度的变化,从而实现对硝酸菌的抑制和淘汰,污泥活性检测表明在该阶段的第55d,SBR活性污泥表现出较高的好氧氨氧化活性[2.91kg·(kg·d)^-1]和极低的亚硝酸盐氧化活性[0.03kg·(kg·d)^-1].厌氧氨氧化菌混合接种阶段,FA浓度通过同时调整进水氨氮浓度和pH进行控制,该阶段由于厌氧氨氧化菌的接种以及渗滤液带人的丰富细菌菌群和营养成分,使SBR的活性污泥不但维持着较高的好氧氨氧化活性[2.83kg·(kg·d)^-1]和一定的亚硝酸盐氧化活性[0.02kg·(kg·d)^-1],并且表现出较高的厌氧氨氧化活性[0.65kg·(kg·d)^-1]和一定的异养反硝化活性[0.11kg·(kg·d)^-1].
Through controlling the concentration of free ammonia in the sequencing batch reactor( SBR), the single-stage autotrophic biological nitrogen removal process was achieved, including partial nitrification and anaerobic ammonium oxidation . The experiment was completed via two steps, the enrichment of nitrite bacteria and the inoculation of the mixture of anammox biomass. The operating temperature in the SBR was(31 ± 2)℃ During the step of the enrichment of nitrite bacteria, pH was about 7.8. Changes of FA concentration were achieved by controlling the concentration of influent NH4+ -N (56-446 mg· L^-1) , in order to inhibit and eliminate the nitrate bacteria. The activity tests of the sludge, 55d after enrichment, showed strong activity of aerobic ammonium oxidation [ 2.91 kg. ( kg. d)^-1 ] and low activity of nitrite oxidation [ 0. 03 kg. ( kg. d) ^-1 ]. During the inoculation of the mixture of anammox biomass, changes of FA concentration were achieved by controlling the concentration of influent NH4+ -N and pH. As the inoculation of anammox biomass , abundant of bacteria and nutrient content were into the reactor and there kept high activity of aerobic ammonium oxidation [ 2.83 kg. ( kg. d)^-1 ] and a certain activity of nitrite oxidation , at the same time , the activity of anammox and heterotrophic denitrification reached 0. 65 kg. ( kg. d)^-1 and 0. 11 kg. ( kg. d)^- 1 , respectively.