中文摘要：

实验制备多孔Si材料作为传感器载体,通过H2O2浸泡的方法使其表面形成Si-OH键,然后对样品进行硅烷化处理。用戊二醛作为偶联剂,通过共价键偶联法将血管紧张素-Ⅱ（Ang-Ⅱ）抗体固定到多孔Si的孔洞中。分别滴加不同浓度的抗原使其发生反应,测量傅里叶变换红外谱（FT-IR）和加入抗体前后反射谱的变化,结果表明,硅烷化效果明显,生物抗原较好固定,抗原抗体反应前后反射谱有明显红移,且红移量于滴加抗原浓度增加而增加,并呈现抗原抗体浓度于红移量的非线性关系。

英文摘要：

The micro-porous silicon is prepared as sensor support material,and the Si-OH band at the surface is formed by the immersion method of hydrogen peroxide.Porous silicon is functionalized by using oxidation and silanization,then Ang-Ⅱ antibody with different concentration are immobilized to the porous surface by glutaraldehyde.The changes of Fourier transformation infrared spectrum and reflect spectrum before and after antigen-antibody reaction are observed.The results show that silanization effect is obvious.After antigen-antibody reaction,the reflection spectrum has a red shift,and the red shift of spectrum increases with the increase of antibody concentration,and presents a nonlinear relationship.