中文摘要：

目的探讨大鼠脊髓全横断损伤后,Wnt信号基因在不同时相脊髓表达的变化及意义。方法成年健康Wistar大鼠30只,随机分为假手术组（sham组）和脊髓损伤组（SCI组）,SCI组分为术后1d、3d、7d、14d、21d组,每组5只。SCI组大鼠T11脊髓段做全横断损伤,sham组仅行椎板切除术,术后大鼠进行BBB运动功能评分。采用半定量RT-PCR方法检测脊髓损伤组织中Wnt信号基因的表达。结果SCI组所有动物在术后均表现为双后肢瘫痪,BBB运动功能评分明显低于sham组。SCI组在脊髓横断术后1d、3d、7d Wnt3a表达均较sham组明显增加;尾侧端Wnt3a较同时相头侧端的表达多,且在21d又出现明显的升高。在sham组和SCI组均未检测到神经基因蛋白1（Ngn1）的表达。发状分裂相关增强子1（Hes1）的表达在SCI后第1d较sham组有明显的下降,第3d出现升高,之后逐渐下降。结论Wnt信号可能参与了脊髓损伤后修复再生的过程。

英文摘要：

Objective To study the changes in the expressions of Wnt signaling gene in spinal cord at different times following spinal cord complete transection injury in rats. Methods Thirty healthy Wistar rats were randomly divided into 2 groups：the sham group （only laminectomy, n = 5） and the spinal cord injury （SCI） group （ n = 25）. The T11 spinal cord segment of adult rats was transected completely in the SCI group. The locomotor functional recovery of hind limbs was observed by grading the Basso-Beattie-Bresnahan（BBB） scale. By using semi-quantitative RT-PCR method, the expression of Wnt signaling gene in the injured tissues of spinal cord was detected. Results The hind limbs of rats were completely paraplyzed in the SCI group and their BBB scales were significantly lower than those in the sham group. In the SCI group, the expression of Wnt3a gene had a remarkable increase both on the rostral and the caudal sides at postoperative days 1, 3, 7; on the caudal side, it was higher than that on the rostral side at the same time and had a second increase at day 21 after the operation. The expression of Ngnl was not detected. Hesl mRNA was significantly decreased at day 1 after the operation, and increased at day 3 and then gradually decreased. ConcIusion Wnt signaling may play an important role in the neural regeneration after SCI.