中文摘要：

目的对唇腭裂跨膜蛋白1样蛋白（CLPTM1L）的表达与肺癌细胞放射敏感性改变的相关性进行研究。方法采用噻唑蓝（M1Tr）方法和细胞克隆形成实验检测细胞的生长和生存率；采用蛋白质印迹（WesternBlot）方法检测蛋白表达水平。结果不同肺癌细胞的放射敏感性与其细胞中CLPTM1L的蛋白表达水平呈负相关，CLPTM1L高表达的肺癌细胞表现出低放射敏感性；提高肺癌细胞中CLPTM1L的表达水平可明显降低γ射线辐照后细胞的放射敏感性，相反转染CLPTM1L小干扰RNA（siRNA）降低CLPTM1L表达水平则大大提高了肺癌细胞的放射治疗敏感性。结论CLPTM1L可能是调节肺癌放射治疗敏感性的一种新靶向基因。

英文摘要：

Objective To study the correlation of cleft lip and palate transmembrane 1 like（CLPTM1L） expression and radiosensitivity of lung cancer cells. Methods Thiazolyl blue tetrazolium bromide（MTT） and cell colony formation assays were used to determine cell growth and survival. Western Blot assay was employed to measure protein expression. Results The results demonstrated a negative correlation between the CLPTM1L expression level and radiosensitivity of lung cancer cells. A lower radiosensitivity in lung cancer cells containing high level of CLPTM1L expression, and vice versa. Enforced expression of CLPTM1L resulted in a significant reduction of radiosensitivity in lung cancer cells irradiated with γ-rays. On the contrary, a marked elevation of radiosensitivity was observed in lung cancer cells transfected with CLPTM1L siRNA. Conclusions CLPTM1L may be a novel target gene in mediating radiosensitivity of lung cancer cells.