试验采用一种自制装置,在负压灌溉原理的基础上,通过设置一系列供水吸力梯度,结合盆栽方式对大豆叶片叶绿素含量、光合及荧光参数的变化进行了研究。结果表明:供水吸力增加,土壤重量含水量下降,大豆叶片叶绿素a(Chla)、叶绿素b(Chlb)含量降低,类胡萝卜紊(Car)含量增加;吸力处理在50~70hPa时光合参数均保持在较高的水平,气孔限制是光合速率(Pn)下降的主要原因,120、140hPa处理时非气孔限制起主要作用;随着光强(PAR)的增加,电子传递速率(ETR)增加,实际光量子产量(Yield)与光化学淬灭(qP)下降,非光化学淬灭(qN)上升,4个处理中50hPa吸力下的大豆叶片活性较高。
This experiment was carried out by using self-deslgned devices for the control of water supply suction. The variety of chlorophyll content and parameters of photosynthetic and chlorophyll fluorescence were studied in this paper. The results showed that with the increase of water supply suction, the content of soil water, the content of Chl a and Chl b all decreased, while the content of carotenoid increased. All the parameters of photosynthesis kept a high level when water supply suction was between 50 hPa and 70 hPa. The main reason for decrease of net photosynthetic rate(Pn) was stomatal limitation under 50-70 hPa, while the nonstomatal limitation was predominant under 120 and 140hPa. With the increase of PAR, Electron transport rate(ETR) and non-photochemistry quenching(qN) increased while actual quantum yield of PSⅡ (Yield) and photochemistry quenching(qP) decreased. The activity of soybean leaves was higher under the condition of 50hPa suction among the four treatments.