研究了实验室条件下刺参(Stichopus japonicus)暴露于不同浓度苯并[a]芘后,刺参体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的变化规律。实验设置了4个处理组(1、5、10、20μg/L)、空白对照组和丙酮溶剂对照组,在染毒2 h、6 h、12 h、24 h、2 d、4 d、7 d、14 d和21 d取样分析,其中第14天放入清洁海水中恢复7 d,分析了苯并[a]芘与刺参SOD、CAT之间的剂量、时间-效应关系。结果表明,在苯并[a]芘作用下,刺参的SOD酶活性在第6小时、12小时和21天时与苯并[a]芘浓度呈显著负相关,R(6h)=0.959 2(P〈0.05)、R(12h)=0.945 8(P〈0.05)、R(21d)=0.959 6(P〈0.05)。刺参CAT的诱导随水体苯并[a]芘浓度的升高而增强,在第2天、第4天有显著的线性关系,
Laboratory study was conducted to determine the effect of benzopyrene on the SOD and CAT activities of Stichopus japonicus. Four test groups containing benzopyrene concentrations of 1,5,10,and 20 μg/L respectively were prepared,with the exposure time of 2 h,6 h,12 h,24 h,2 d,4 d,7 d,14 d and 21 d,among others,the Stichopus japonicus sample with 14 d-exposure were placed in clean seawater for seven days. Findings of the study indicated that :SOD activities of Stichopus japonicus and benzopyrene concentrations were correlated negatively at 6 h,12 h and 21 d(R(6h)=0.959 2(P〈0.05),R(12h)=0.945 8(P〈0.05),R(21d)=0.959 6(P〈0.05); CAT activities of Stichopus japonicus and benzopyrene concentrations were significantly correlated at 2 d and 4 d(R(2d)=0.790 6(0.01 P〈0.05),and R(4d)=0.953 3(0.01P〈0.05). In conclusion,under the stress of benzopyrene toxicity,SOD and CAT were characterized with remarkable dose-time effect,and SOD and CAT activities of Stichopus japonicus could be chosen as a bio-marker to evaluate the pollution level of benzopyrene.