中文摘要：

目的：比较不同来源狼毒药材及醋炙前后的质量差异。方法：以岩大戟内酯B为参照物,采用UPLC法,使用Waters ODS色谱柱（100 mm×2.1 mm,1.8μm）,乙腈-水梯度洗脱,流速0.4 m L/min,柱温30℃,检测波长210 nm,建立了月腺大戟来源的狼毒药材、醋炙饮片及狼毒大戟来源的狼毒药材的UPLC指纹图谱,分析时间不超过20 min。结果：药材或饮片的批次间的相似度均大于0.90,质量稳定;同一来源狼毒醋炙后共有峰减少,且个别成分相对含量有不同程度的升高或降低;狼毒大戟与月腺大戟来源的狼毒药材之间相似度小于0.72,差异显著。结论：该研究为完善狼毒的质量控制与阐述其醋炙机理提供了依据。

英文摘要：

Objective： To compare the qualities of Euphorbiae Ebracteolatae Radix from different original plants and its vinegarprocessed slices.Methods： UPLC fingerprints of Euphoria ebracteolata,Euphoria fischeriana and vinegar-processed slices of Euphoria ebracteolata were established on a Waters ODS column（ 100 mm × 2.1 mm,1.8 μm）,using jolkinolide B as the reference substance.A mixture of acetonitrile and water was used as the mobile phase in a gradient elution mode,with the flow rate of 0.4 m L / min,the column temperature at 30 ℃ and the UV detector at 210 nm.The UPLC analysis time was no more than 20 min.Results： The qualities of Euphoria ebracteolata and its vinegar-processed slices were stable with similarities over 0.90,respectively.However,the fingerprints of the slices and their original crude drugs exhibited obvious difference on both number and the relative peak area of the common peaks.Meanwhile the similarities of the fingerprints of Euphoria fischeriana and Euphoria ebracteolata were less than 0.72,indicating significant difference of these two plants.Conclusion： The developed method is available for quality control of Euphorbiae Ebracteolatae Radix and mechanism exploration of its vinegar-processed slices.